1s5h
From Proteopedia
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'''Potassium Channel Kcsa-Fab Complex T75C mutant in K+''' | '''Potassium Channel Kcsa-Fab Complex T75C mutant in K+''' | ||
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[[Category: Mackinnon, R.]] | [[Category: Mackinnon, R.]] | ||
[[Category: Zhou, M.]] | [[Category: Zhou, M.]] | ||
| - | [[Category: | + | [[Category: Ion occupancy]] |
| - | [[Category: | + | [[Category: K+ channel]] |
| - | [[Category: | + | [[Category: Protein-antibody fab complex]] |
| - | [[Category: | + | [[Category: Selectivity filter]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:19:31 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:19, 3 May 2008
Potassium Channel Kcsa-Fab Complex T75C mutant in K+
Overview
The selectivity filter of K(+) channels is comprised of a linear queue of four equal-spaced ion-binding sites spanning a distance of 12A. Each site is formed of eight oxygen atoms from the protein. The first three sites, numbered 1-3 from the extracellular side, are made of exclusively main-chain carbonyl oxygen atoms. The fourth site, closest to the intracellular side, is made of four main-chain carbonyl oxygen atoms and four threonine side-chain hydroxyl oxygen atoms. Here we characterize the effects of mutating the threonine to cysteine on the distribution of ions in the selectivity filter and on the conduction of ions through the filter. The mutation influences the occupancy of K(+) at sites 2 and 4 and it reduces the maximum rate of conduction in the limit of high K(+) concentration. The mutation does not affect the conduction of Rb(+). These results can be understood in the context of a conduction mechanism in which a pair of K ions switch between energetically balanced 1,3 and 2,4 configurations.
About this Structure
1S5H is a Single protein structure of sequence from Mus musculus and Streptomyces coelicolor, and streptomyces lividans. Full crystallographic information is available from OCA.
Reference
A mutant KcsA K(+) channel with altered conduction properties and selectivity filter ion distribution., Zhou M, MacKinnon R, J Mol Biol. 2004 May 7;338(4):839-46. PMID:15099749 Page seeded by OCA on Sat May 3 08:19:31 2008
