1s67
From Proteopedia
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'''Crystal structure of heme domain of direct oxygen sensor from E. coli''' | '''Crystal structure of heme domain of direct oxygen sensor from E. coli''' | ||
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[[Category: Satterlee, J D.]] | [[Category: Satterlee, J D.]] | ||
[[Category: Suquet, C.]] | [[Category: Suquet, C.]] | ||
| - | [[Category: | + | [[Category: E coli]] |
| - | [[Category: | + | [[Category: Heme]] |
| - | [[Category: | + | [[Category: Oxygen storage/transport]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:21:11 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:21, 3 May 2008
Crystal structure of heme domain of direct oxygen sensor from E. coli
Overview
The X-ray crystal structure of the Escherichia coli (Ec) direct oxygen sensor heme domain (Ec DosH) has been solved to 1.8 A using Fe multiple-wavelength anomalous dispersion (MAD), and the positions of Met95 have been confirmed by selenomethionine ((Se)Met) MAD. Ec DosH is the sensing part of a larger two-domain sensing/signaling protein, in which the signaling domain has phosphodiesterase activity. The asymmetric unit of the crystal lattice contains a dimer comprised of two differently ligated heme domain monomers. Except for the heme ligands, the monomer heme domains are identical. In one monomer, the heme is ligated by molecular oxygen (O(2)), while in the other monomer, an endogenous Met95 with S --> Fe ligation replaces the exogenous O(2) ligand. In both heme domains, the proximal ligand is His77. Analysis of these structures reveals sizable ligand-dependent conformational changes in the protein chain localized in the FG turn, the G(beta)-strand, and the HI turn. These changes provide insight to the mechanism of signal propagation within the heme domain following initiation due to O(2) dissociation.
About this Structure
1S67 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Insights into signal transduction involving PAS domain oxygen-sensing heme proteins from the X-ray crystal structure of Escherichia coli Dos heme domain (Ec DosH)., Park H, Suquet C, Satterlee JD, Kang C, Biochemistry. 2004 Mar 16;43(10):2738-46. PMID:15005609 Page seeded by OCA on Sat May 3 08:21:11 2008
