8xje
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the YqeY protein from Campylobacter jejuni== | |
| + | <StructureSection load='8xje' size='340' side='right'caption='[[8xje]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8xje]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XJE FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xje OCA], [https://pdbe.org/8xje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xje RCSB], [https://www.ebi.ac.uk/pdbsum/8xje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xje ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | YqeY is a functionally and structurally uncharacterized protein that is ubiquitously expressed in bacteria. To gain structural insights into the function of YqeY, we determined the crystal structures of the Campylobacter jejuni and Vibrio parahaemolyticus YqeY proteins (cjYqeY and vpYqeY, respectively) and analyzed the structural and functional roles of conserved residues via a mutational study. Both cjYqeY and vpYqeY were found to adopt a two-domain structure consisting of an N-terminal four-alpha-helix domain and a C-terminal three-alpha-helix domain, with a relatively flexible interdomain orientation. The YqeY structure is unique in its linkage of the two alpha-helix domains although the C-terminal YqeY domain is structurally homologous to the terminal appendages of glutaminyl-tRNA synthetase and tRNA-dependent amidotransferase. We identified six conserved YqeY residues (Y67, R72, E82, Y89, P91, and G119) and evaluated their roles in protein stability via alanine mutation using a thermal shift assay. Residues Y67, R72, Y89, and P91 were shown to be required to maintain the structural integrity of YqeY. In contrast, residues E82 and G119 were not found to be essential for protein stability and are highly likely to contribute to the biological function of YqeY. | ||
| - | + | Structural analysis of the YqeY proteins from Campylobacter jejuni and Vibrio parahaemolyticus.,Kim SY, Yoon SI Biochem Biophys Res Commun. 2024 Feb 5;695:149485. doi: , 10.1016/j.bbrc.2024.149485. Epub 2024 Jan 5. PMID:38211535<ref>PMID:38211535</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8xje" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Campylobacter jejuni]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kim SY]] | ||
| + | [[Category: Yoon SI]] | ||
Current revision
Crystal structure of the YqeY protein from Campylobacter jejuni
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