1v3q

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Revision as of 17:34, 12 November 2007

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spinqualitylabelsall models 1v3q, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Structure of human PNP complexed with DDI

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Human purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme which, plays a key role in the purine salvage pathway, and PNP deficiency in, humans leads to an impairment of T-cell function, usually with no apparent, effect on B-cell function. PNP is highly specific for 6-oxopurine, nucleosides and exhibits negligible activity for 6-aminopurine, nucleosides. The catalytic efficiency for inosine is 350,000-fold greater, than for adenosine. Adenine nucleosides and nucleotides are deaminated by, adenosine deaminase and AMP deaminase to their corresponding inosine, derivatives which, in turn, may be further degraded. Here we report the, crystal structures of human PNP in complex with inosine and, 2('),3(')-dideoxyinosine, refined to 2.8A resolution using synchrotron, radiation. The present structures provide explanation for ligand binding, refine the purine-binding site, and can be used for future inhibitor, design.

Disease

Known diseases associated with this structure: Neutral lipid storage disease with myopathy OMIM:[609059], Nucleoside phosphorylase deficiency, immunodeficiency due to OMIM:[164050]

About this Structure

1V3Q is a Single protein structure of sequence from Homo sapiens with SO4 and 2DI as ligands. Active as Purine-nucleoside phosphorylase, with EC number 2.4.2.1 Full crystallographic information is available from OCA.

Reference

Structures of human purine nucleoside phosphorylase complexed with inosine and ddI., Canduri F, dos Santos DM, Silva RG, Mendes MA, Basso LA, Palma MS, de Azevedo WF, Santos DS, Biochem Biophys Res Commun. 2004 Jan 23;313(4):907-14. PMID:14706628

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