1s7f
From Proteopedia
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'''RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)''' | '''RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)''' | ||
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[[Category: Roderick, S L.]] | [[Category: Roderick, S L.]] | ||
[[Category: Vetting, M W.]] | [[Category: Vetting, M W.]] | ||
| - | [[Category: | + | [[Category: Acetyltransferase]] |
| - | [[Category: | + | [[Category: Alpha-n-protein acetyltransferase]] |
| - | [[Category: | + | [[Category: Coenzyme some]] |
| - | [[Category: | + | [[Category: Gnat]] |
| - | [[Category: | + | [[Category: L7/l12]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:23:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:23, 3 May 2008
RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)
Overview
RimL is responsible for converting the prokaryotic ribosomal protein from L12 to L7 by acetylation of its N-terminal amino group. We demonstrate that purified RimL is capable of posttranslationally acetylating L12, exhibiting a V(max) of 21 min(-1). We have also determined the apostructure of RimL from Salmonella typhimurium and its complex with coenzyme A, revealing a homodimeric oligomer with structural similarity to other Gcn5-related N-acetyltransferase superfamily members. A large central trough located at the dimer interface provides sufficient room to bind both L12 N-terminal helices. Structural and biochemical analysis indicates that RimL proceeds by single-step transfer rather than a covalent-enzyme intermediate. This is the first structure of a Gcn5-related N-acetyltransferase family member with demonstrated activity toward a protein N(alpha)-amino group and is a first step toward understanding the molecular basis for N(alpha)acetylation and its function in cellular regulation.
About this Structure
1S7F is a Single protein structure of sequence from Salmonella typhimurium lt2. Full crystallographic information is available from OCA.
Reference
A novel dimeric structure of the RimL Nalpha-acetyltransferase from Salmonella typhimurium., Vetting MW, de Carvalho LP, Roderick SL, Blanchard JS, J Biol Chem. 2005 Jun 10;280(23):22108-14. Epub 2005 Apr 6. PMID:15817456 Page seeded by OCA on Sat May 3 08:23:29 2008
