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| | ==NMR Structure of RRM-2 of Yeast NPL3 Protein== | | ==NMR Structure of RRM-2 of Yeast NPL3 Protein== |
| - | <StructureSection load='2osr' size='340' side='right'caption='[[2osr]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2osr' size='340' side='right'caption='[[2osr]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2osr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OSR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OSR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2osr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OSR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OSR FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2osq|2osq]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NOP3, MTS1, NAB1, NPL3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2osr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2osr OCA], [https://pdbe.org/2osr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2osr RCSB], [https://www.ebi.ac.uk/pdbsum/2osr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2osr ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2osr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2osr OCA], [https://pdbe.org/2osr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2osr RCSB], [https://www.ebi.ac.uk/pdbsum/2osr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2osr ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/NOP3_YEAST NOP3_YEAST]] Required for pre-rRNA processing and nuclear import as well as mitochondrial protein targeting. Binds to poly(A).
| + | [https://www.uniprot.org/uniprot/NOP3_YEAST NOP3_YEAST] Required for pre-rRNA processing and nuclear import as well as mitochondrial protein targeting. Binds to poly(A). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Allain, F H]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Bucheli, M]] | + | [[Category: Allain FH]] |
| - | [[Category: Buratowski, S]] | + | [[Category: Bucheli M]] |
| - | [[Category: Deka, P]] | + | [[Category: Buratowski S]] |
| - | [[Category: Moore, C]] | + | [[Category: Deka P]] |
| - | [[Category: Skrisovska, L]] | + | [[Category: Moore C]] |
| - | [[Category: Varani, G]] | + | [[Category: Skrisovska L]] |
| - | [[Category: Mrna]]
| + | [[Category: Varani G]] |
| - | [[Category: Npl3]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rna-binding]]
| + | |
| - | [[Category: Rrm]]
| + | |
| - | [[Category: Sr protein]]
| + | |
| Structural highlights
Function
NOP3_YEAST Required for pre-rRNA processing and nuclear import as well as mitochondrial protein targeting. Binds to poly(A).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Yeast Npl3 is homologous to SR proteins in higher eukaryotes, a family of RNA-binding proteins that have multiple essential roles in RNA metabolism. This protein competes with 3'-end processing factors for binding to the nascent RNA, protecting the transcript from premature termination and coordinating transcription termination and the packaging of the fully processed transcript for export. The NMR structure of its RNA-binding domain shows two unusually compact RNA recognition motifs (RRMs), and identifies the RNA recognition surface in Npl3. Biochemical and NMR studies identify a class of G+U-rich RNA sequences with high specificity for this protein. The protein binds to RNA and forms a single globular structure, but the two RRMs of Npl3 are not equivalent, with the second domain forming much stronger interactions with G+U-rich RNA sequences that occur independently of the interaction of the first RRM. The specific binding to G+U-rich RNAs observed for the two RRMs of Npl3 is masked in the full-length protein by a much stronger but non-sequence-specific RNA-binding activity residing outside of its RRMs. The preference of Npl3 for G+U-rich sequences supports the model for its function in regulating recognition of 3'-end processing sites through competition with the Rna15 (yeast analog of human CstF-64 protein) subunit of the processing complex.
Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals.,Deka P, Bucheli ME, Moore C, Buratowski S, Varani G J Mol Biol. 2008 Jan 4;375(1):136-50. Epub 2007 Sep 16. PMID:18022637[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Deka P, Bucheli ME, Moore C, Buratowski S, Varani G. Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals. J Mol Biol. 2008 Jan 4;375(1):136-50. Epub 2007 Sep 16. PMID:18022637 doi:10.1016/j.jmb.2007.09.029
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