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| | <SX load='6rxx' size='340' side='right' viewer='molstar' caption='[[6rxx]], [[Resolution|resolution]] 7.10Å' scene=''> | | <SX load='6rxx' size='340' side='right' viewer='molstar' caption='[[6rxx]], [[Resolution|resolution]] 7.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6rxx]] is a 68 chain structure with sequence from [http://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RXX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6RXX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6rxx]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RXX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RXX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.1Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6rxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rxx OCA], [http://pdbe.org/6rxx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rxx RCSB], [http://www.ebi.ac.uk/pdbsum/6rxx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rxx ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rxx OCA], [https://pdbe.org/6rxx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rxx RCSB], [https://www.ebi.ac.uk/pdbsum/6rxx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rxx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/G0S3V7_CHATD G0S3V7_CHATD]] Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.[PIRNR:PIRNR006515] [[http://www.uniprot.org/uniprot/PNO1_CHATD PNO1_CHATD]] Required for small ribosomal subunit (SSU) synthesis. Has a role in the processing of early nucleolar and late cytoplasmic pre-RNA species.[UniProtKB:Q99216] [[http://www.uniprot.org/uniprot/NOL10_CHATD NOL10_CHATD]] May be involved in rRNA-processing and ribosome biosynthesis.[UniProtKB:P48234] [[http://www.uniprot.org/uniprot/UTP11_CHATD UTP11_CHATD]] Involved in nucleolar processing of pre-18S ribosomal RNA.[UniProtKB:P34247] | + | [https://www.uniprot.org/uniprot/G0SF93_CHATD G0SF93_CHATD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| | *[[Ribosome 3D structures|Ribosome 3D structures]] | | *[[Ribosome 3D structures|Ribosome 3D structures]] |
| | + | *[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | [[Category: Chaetomium thermophilum]] | | [[Category: Chaetomium thermophilum]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Beckmann, R]] | + | [[Category: Beckmann R]] |
| - | [[Category: Berninghausen, O]] | + | [[Category: Berninghausen O]] |
| - | [[Category: Cheng, J]] | + | [[Category: Cheng J]] |
| - | [[Category: Griesel, S]] | + | [[Category: Griesel S]] |
| - | [[Category: Hurt, E]] | + | [[Category: Hurt E]] |
| - | [[Category: Kellner, N]] | + | [[Category: Kellner N]] |
| - | [[Category: Ribosome]]
| + | |
| - | [[Category: Ribosome biogenesis]]
| + | |
| - | [[Category: Rrna]]
| + | |
| Structural highlights
Function
G0SF93_CHATD
Publication Abstract from PubMed
Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5' > 3', but this directionality may not be accurate. Here, by analyzing the structure of a series of 90S assembly intermediates from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. Large parts of the 18S rRNA 3' and central domains assemble first into the 90S before the 5' domain is integrated. This final incorporation depends on a contact between a heterotrimer Enp2-Bfr2-Lcp5 recruited to the flexible 5' domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S. Keeping the 5' domain temporarily segregated from the 90S scaffold could provide extra time to complete the multifaceted 5' domain folding, which depends on a distinct set of snoRNAs and processing factors.
Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration.,Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E Mol Cell. 2019 Jul 15. pii: S1097-2765(19)30489-7. doi:, 10.1016/j.molcel.2019.06.032. PMID:31378463[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E. Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration. Mol Cell. 2019 Jul 15. pii: S1097-2765(19)30489-7. doi:, 10.1016/j.molcel.2019.06.032. PMID:31378463 doi:http://dx.doi.org/10.1016/j.molcel.2019.06.032
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