1s97

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[[Image:1s97.gif|left|200px]]
[[Image:1s97.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1s97 |SIZE=350|CAPTION= <scene name='initialview01'>1s97</scene>, resolution 2.40&Aring;
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The line below this paragraph, containing "STRUCTURE_1s97", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DCT:2&#39;,3&#39;-DIDEOXYCYTIDINE+5&#39;-TRIPHOSPHATE'>DCT</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= DBH, DPO4, SSO2448 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])
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-->
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|DOMAIN=
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{{STRUCTURE_1s97| PDB=1s97 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s97 OCA], [http://www.ebi.ac.uk/pdbsum/1s97 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s97 RCSB]</span>
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}}
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'''DPO4 with GT mismatch'''
'''DPO4 with GT mismatch'''
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[[Category: Trincao, J.]]
[[Category: Trincao, J.]]
[[Category: Wolfle, W T.]]
[[Category: Wolfle, W T.]]
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[[Category: dna duplex]]
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[[Category: Dna duplex]]
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[[Category: g t mismatch]]
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[[Category: G t mismatch]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:26:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:38:58 2008''
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Revision as of 05:26, 3 May 2008

Image:1s97.gif

Template:STRUCTURE 1s97

DPO4 with GT mismatch


Overview

The ability or inability of a DNA polymerase to extend a mispair directly affects the establishment of genomic mutations. We report here kinetic analyses of the ability of Dpo4, a Y-family polymerase from Sulfolobus solfataricus, to extend from all mispairs opposite a template G or T. Dpo4 is equally inefficient at extending these mispairs, which include, surprisingly, a G.T mispair expected to conform closely to Watson-Crick geometry. To elucidate the basis of this, we solved the structure of Dpo4 bound to G.T-mispaired primer template in the presence of an incoming nucleotide. As a control, we also determined the structure of Dpo4 bound to a matched A-T base pair at the primer terminus. The structures offer a basis for the low efficiency of Dpo4 in extending a G.T mispair: a reverse wobble that deflects the primer 3'-OH away from the incoming nucleotide.

About this Structure

1S97 is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

Dpo4 is hindered in extending a G.T mismatch by a reverse wobble., Trincao J, Johnson RE, Wolfle WT, Escalante CR, Prakash S, Prakash L, Aggarwal AK, Nat Struct Mol Biol. 2004 May;11(5):457-62. Epub 2004 Apr 11. PMID:15077104 Page seeded by OCA on Sat May 3 08:26:15 2008

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OCA

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