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| | ==Solution Structure of Ca2+-bound DdCAD-1== | | ==Solution Structure of Ca2+-bound DdCAD-1== |
| - | <StructureSection load='2b1o' size='340' side='right'caption='[[2b1o]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2b1o' size='340' side='right'caption='[[2b1o]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2b1o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dicdi Dicdi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B1O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2b1o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B1O FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1yhp|1yhp]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cadA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])</td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b1o OCA], [https://pdbe.org/2b1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b1o RCSB], [https://www.ebi.ac.uk/pdbsum/2b1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b1o ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b1o OCA], [https://pdbe.org/2b1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b1o RCSB], [https://www.ebi.ac.uk/pdbsum/2b1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b1o ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/CAD1_DICDI CAD1_DICDI]] Mediates calcium-dependent cell-cell adhesion during the early stage of development.<ref>PMID:8663243</ref>
| + | [https://www.uniprot.org/uniprot/CAD1_DICDI CAD1_DICDI] Mediates calcium-dependent cell-cell adhesion during the early stage of development.<ref>PMID:8663243</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Dicdi]] | + | [[Category: Dictyostelium discoideum]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Huang, H B]] | + | [[Category: Huang HB]] |
| - | [[Category: Lin, Z]] | + | [[Category: Lin Z]] |
| - | [[Category: Siu, C H]] | + | [[Category: Siu CH]] |
| - | [[Category: Sriskanthadevan, S]] | + | [[Category: Sriskanthadevan S]] |
| - | [[Category: Yang, D W]] | + | [[Category: Yang DW]] |
| - | [[Category: Calcium]]
| + | |
| - | [[Category: Cell adherison]]
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| - | [[Category: Cell adhesion]]
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| - | [[Category: Ddcad-1]]
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| Structural highlights
Function
CAD1_DICDI Mediates calcium-dependent cell-cell adhesion during the early stage of development.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
DdCAD-1 is a novel Ca(2+)-dependent cell adhesion molecule that lacks a hydrophobic signal peptide and a transmembrane domain. DdCAD-1 is expressed by the social amoeba Dictyostelium discoideum at the onset of development. It is synthesized as a soluble protein and then transported to the plasma membrane by contractile vacuoles. Here we describe the novel features of the solution structures of Ca(2+)-free and Ca(2+)-bound monomeric DdCAD-1. DdCAD-1 contains two beta-sandwich domains, belonging to the betagamma-crystallin and immunoglobulin fold classes, respectively. Whereas the N-terminal domain has a major role in homophilic binding, the C-terminal domain tethers the protein to the cell membrane. From structural and mutational analyses, we propose a model for the Ca(2+)-bound DdCAD-1 dimer as a basis for understanding DdCAD-1-mediated cell-cell adhesion at the molecular level. Our results provide new insights into Ca(2+)-dependent mechanisms for cell-cell adhesion.
Solution structures of the adhesion molecule DdCAD-1 reveal new insights into Ca(2+)-dependent cell-cell adhesion.,Lin Z, Sriskanthadevan S, Huang H, Siu CH, Yang D Nat Struct Mol Biol. 2006 Nov;13(11):1016-22. Epub 2006 Oct 22. PMID:17057715[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wong EF, Brar SK, Sesaki H, Yang C, Siu CH. Molecular cloning and characterization of DdCAD-1, a Ca2+-dependent cell-cell adhesion molecule, in Dictyostelium discoideum. J Biol Chem. 1996 Jul 5;271(27):16399-408. PMID:8663243
- ↑ Lin Z, Sriskanthadevan S, Huang H, Siu CH, Yang D. Solution structures of the adhesion molecule DdCAD-1 reveal new insights into Ca(2+)-dependent cell-cell adhesion. Nat Struct Mol Biol. 2006 Nov;13(11):1016-22. Epub 2006 Oct 22. PMID:17057715 doi:10.1038/nsmb1162
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