1s9r

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[[Image:1s9r.gif|left|200px]]
[[Image:1s9r.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1s9r |SIZE=350|CAPTION= <scene name='initialview01'>1s9r</scene>, resolution 1.60&Aring;
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The line below this paragraph, containing "STRUCTURE_1s9r", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Arginine_deiminase Arginine deiminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.6 3.5.3.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1s9r| PDB=1s9r | SCENE= }}
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|RELATEDENTRY=[[1lxy|1LXY]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s9r OCA], [http://www.ebi.ac.uk/pdbsum/1s9r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s9r RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF ARGININE DEIMINASE COVALENTLY LINKED WITH A REACTION INTERMEDIATE'''
'''CRYSTAL STRUCTURE OF ARGININE DEIMINASE COVALENTLY LINKED WITH A REACTION INTERMEDIATE'''
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[[Category: 5-fold pseudo-symmetric domain]]
[[Category: 5-fold pseudo-symmetric domain]]
[[Category: 5-helix bundle domain]]
[[Category: 5-helix bundle domain]]
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[[Category: deiminase]]
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[[Category: Deiminase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: raction intermediate]]
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[[Category: Raction intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:27:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:39:12 2008''
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Revision as of 05:27, 3 May 2008

Image:1s9r.gif

Template:STRUCTURE 1s9r

CRYSTAL STRUCTURE OF ARGININE DEIMINASE COVALENTLY LINKED WITH A REACTION INTERMEDIATE


Overview

Arginine deiminase (ADI), an enzyme that hydrolyzes arginine to generate energy in many parasitic microorganisms, has potent anticancer activities and can halt growth of solid tumors. We determined the crystal structure of ADI from Mycoplasma arginini in two different forms (1.6 and 2.0 A resolution) using multiple isomorphous replacement. ADI shares common structural features with the arginine-catabolizing enzymes Arg:Gly amidinotransferase and dimethylarginine dimethyl-aminohydrolase; ADI contains an additional domain of five helices. The scissile C-N bonds of the substrates and the catalytic triads (Cys398-His269-Glu213 of ADI) for the three enzymes superimpose on each other. The ADI structure from form I crystals corresponds to a tetrahedral intermediate with four heteroatoms (1S, 2N, 1O) covalently bonded to the reaction-center carbon. The structure from form II crystals represents an amidino-enzyme complex; the reaction-center carbon is covalently bonded to Cys398 sulfur and two nitrogens, and the reacting water molecule is only 2.54 A away.

About this Structure

1S9R is a Single protein structure of sequence from Mycoplasma arginini. Full crystallographic information is available from OCA.

Reference

Crystal structures of arginine deiminase with covalent reaction intermediates; implications for catalytic mechanism., Das K, Butler GH, Kwiatkowski V, Clark AD Jr, Yadav P, Arnold E, Structure. 2004 Apr;12(4):657-67. PMID:15062088 Page seeded by OCA on Sat May 3 08:27:41 2008

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