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1s9s
From Proteopedia
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[[Image:1s9s.gif|left|200px]] | [[Image:1s9s.gif|left|200px]] | ||
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'''SOLUTION STRUCTURE OF MLV PSI SITE''' | '''SOLUTION STRUCTURE OF MLV PSI SITE''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9S OCA]. | |
==Reference== | ==Reference== | ||
NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus., D'Souza V, Dey A, Habib D, Summers MF, J Mol Biol. 2004 Mar 19;337(2):427-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15003457 15003457] | NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus., D'Souza V, Dey A, Habib D, Summers MF, J Mol Biol. 2004 Mar 19;337(2):427-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15003457 15003457] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Dey, A.]] | [[Category: Dey, A.]] | ||
[[Category: Habib, D.]] | [[Category: Habib, D.]] | ||
[[Category: Souza, V D.]] | [[Category: Souza, V D.]] | ||
[[Category: Summers, M F.]] | [[Category: Summers, M F.]] | ||
| - | [[Category: | + | [[Category: A-c mismatch]] |
| - | [[Category: | + | [[Category: A-minor k-turn]] |
| - | [[Category: | + | [[Category: Bulge]] |
| - | [[Category: | + | [[Category: G-a mismatch]] |
| - | [[Category: | + | [[Category: G-u mismatch]] |
| - | [[Category: | + | [[Category: Mlv]] |
| - | [[Category: | + | [[Category: Stem loop]] |
| - | [[Category: | + | [[Category: U-u mismatch]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:27:44 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:27, 3 May 2008
SOLUTION STRUCTURE OF MLV PSI SITE
Overview
The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly. We have determined the structure of a 101-nucleotide RNA that contains this signal (called mPsi) using a novel isotope-edited NMR approach. The method is robust and should be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide linker. The structure contains five mismatched base-pairs, an unusual C.CG base-triple platform, and a novel "A-minor K-turn," in which unpaired adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond with the phosphodiester of A341. Phylogenetic analyses indicate that these essential structural elements are conserved among the murine C-type retroviruses.
About this Structure
Full crystallographic information is available from OCA.
Reference
NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus., D'Souza V, Dey A, Habib D, Summers MF, J Mol Biol. 2004 Mar 19;337(2):427-42. PMID:15003457 Page seeded by OCA on Sat May 3 08:27:44 2008
Categories: Dey, A. | Habib, D. | Souza, V D. | Summers, M F. | A-c mismatch | A-minor k-turn | Bulge | G-a mismatch | G-u mismatch | Mlv | Stem loop | U-u mismatch
