2cbp

From Proteopedia

(Difference between revisions)

Current revision

CUCUMBER BASIC PROTEIN, A BLUE COPPER PROTEIN

Structural highlights

2cbp is a 1 chain structure with sequence from Cucumis sativus. This structure supersedes the now removed PDB entry 1cbp. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BABL_CUCSA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of the cucumber basic protein (CBP), a type 1 or blue copper protein, has been refined at 1.8 A resolution. The molecule resembles other blue copper proteins in having a Greek key beta-barrel structure, except that the barrel is open on one side and is better described as a "beta-sandwich" or "beta-taco". The Cu atom has the normal blue copper NNSS' co-ordination with bond lengths Cu-N(His39) = 1.93 A, Cu-S(Cys79) = 2.16 A, Cu-N(His84) = 1.95 A, Cu-S(Met89) = 2.61 A. The Cu-S(Met) bond is the shortest so far observed in a blue copper protein. A disulphide link, (Cys52)-S-S-(Cys85), appears to play an important role in stabilising the molecular structure. It is suggested that the polypeptide fold is typical of a sub-family of blue copper proteins (phytocyanins) as well as a non-metalloprotein, ragweed allergen Ra3, with which CBP has a high degree of sequence identify. The proteins currently identifiable as phytocyanins are CBP, stellacyanin, mavicyanin, umecyanin, a cucumber peeling cupredoxin, a putative blue copper protein in pea pods, and a blue copper protein from Arabidopsis thaliana. In all except CBP and the pea-pod protein, the axial methionine ligand normally found at blue copper sites is replaced by glutamine. The structure of CBP was originally solved by the multiple wavelength anomalous scattering method, using data recorded at four wavelengths. All these data were included in the restrained least squares refinement. The final model comprises 96 amino acid residues, 122 solvent molecules and a copper atom. Several residues are modelled as having more than one conformation. The residual R is 0.141 for 41,910 observations (including Bijvoet-related observations) of 8.142 unique reflections in the resolution range 7 to 1.8 A.

The structure of a phytocyanin, the basic blue protein from cucumber, refined at 1.8 A resolution.,Guss JM, Merritt EA, Phizackerley RP, Freeman HC J Mol Biol. 1996 Oct 11;262(5):686-705. PMID:8876647^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Guss JM, Merritt EA, Phizackerley RP, Freeman HC. The structure of a phytocyanin, the basic blue protein from cucumber, refined at 1.8 A resolution. J Mol Biol. 1996 Oct 11;262(5):686-705. PMID:8876647 doi:10.1006/jmbi.1996.0545

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2cbp"

Categories: Cucumis sativus | Large Structures | Freeman HC | Guss JM

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2cbp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cucumis_sativus Cucumis sativus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cbp 1cbp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CBP FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbp OCA], [https://pdbe.org/2cbp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cbp RCSB], [https://www.ebi.ac.uk/pdbsum/2cbp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cbp ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/BABL_CUCSA BABL_CUCSA]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 35: @@
 [[Category: Cucumis sativus]]
 [[Category: Large Structures]]
-[[Category: Freeman, H C]]
+[[Category: Freeman HC]]
-[[Category: Guss, J M]]
+[[Category: Guss JM]]
-[[Category: Electron transport]]
-[[Category: Phytocyanin]]
-[[Category: Type 1 copper protein]]

2cbp

From Proteopedia

Current revision

CUCUMBER BASIC PROTEIN, A BLUE COPPER PROTEIN

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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