1sa0
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1sa0.gif|left|200px]] | [[Image:1sa0.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1sa0", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1sa0| PDB=1sa0 | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX''' | '''TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX''' | ||
| Line 33: | Line 30: | ||
[[Category: Ravelli, R B.]] | [[Category: Ravelli, R B.]] | ||
[[Category: Sobel, A.]] | [[Category: Sobel, A.]] | ||
| - | [[Category: | + | [[Category: Alpha-tubulin]] |
| - | [[Category: | + | [[Category: Beta-tubulin]] |
| - | [[Category: | + | [[Category: Colchicine]] |
| - | [[Category: | + | [[Category: Gtpase]] |
| - | [[Category: | + | [[Category: Microtubule podophyllotoxin]] |
| - | [[Category: | + | [[Category: Stathmin]] |
| - | [[Category: | + | [[Category: Tubulin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:28:14 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:28, 3 May 2008
TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX
Overview
Microtubules are cytoskeletal polymers of tubulin involved in many cellular functions. Their dynamic instability is controlled by numerous compounds and proteins, including colchicine and stathmin family proteins. The way in which microtubule instability is regulated at the molecular level has remained elusive, mainly because of the lack of appropriate structural data. Here, we present the structure, at 3.5 A resolution, of tubulin in complex with colchicine and with the stathmin-like domain (SLD) of RB3. It shows the interaction of RB3-SLD with two tubulin heterodimers in a curved complex capped by the SLD amino-terminal domain, which prevents the incorporation of the complexed tubulin into microtubules. A comparison with the structure of tubulin in protofilaments shows changes in the subunits of tubulin as it switches from its straight conformation to a curved one. These changes correlate with the loss of lateral contacts and provide a rationale for the rapid microtubule depolymerization characteristic of dynamic instability. Moreover, the tubulin-colchicine complex sheds light on the mechanism of colchicine's activity: we show that colchicine binds at a location where it prevents curved tubulin from adopting a straight structure, which inhibits assembly.
About this Structure
1SA0 is a Protein complex structure of sequences from Bos taurus and Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain., Ravelli RB, Gigant B, Curmi PA, Jourdain I, Lachkar S, Sobel A, Knossow M, Nature. 2004 Mar 11;428(6979):198-202. PMID:15014504 Page seeded by OCA on Sat May 3 08:28:14 2008
