1scd
From Proteopedia
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'''X-RAY CRYSTAL STRUCTURE OF CROSS-LINKED SUBTILISM CARLSBERG IN WATER VS. ACETONITRILE''' | '''X-RAY CRYSTAL STRUCTURE OF CROSS-LINKED SUBTILISM CARLSBERG IN WATER VS. ACETONITRILE''' | ||
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[[Category: Klibanov, A M.]] | [[Category: Klibanov, A M.]] | ||
[[Category: Ringe, D.]] | [[Category: Ringe, D.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:32:32 2008'' | |
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Revision as of 05:32, 3 May 2008
X-RAY CRYSTAL STRUCTURE OF CROSS-LINKED SUBTILISM CARLSBERG IN WATER VS. ACETONITRILE
Overview
The crystal structure of subtilisin Carlsberg lightly cross-linked with glutaraldehyde was solved in aqueous solution by X-ray crystallography at 2.3 A resolution. It was found to be virtually identical to the recently determined (Fitzpatrick, P.A., Steinmetz, A.C.U., Ringe, D.A. & Klibanov, A.M. (1993) Proc. Natl. Acad. Sci. USA 90, 8653) structure of the cross-linked enzyme in anhydrous acetonitrile. The latter structure was found to be significantly more rigid than in water, as reflected by their average B factors. The numbers of subtilisin-bound water molecules in the two structures are similar (114 and 99 in water and in acetonitrile, respectively), but the locations of some half of these bound waters are distinct.
About this Structure
1SCD is a Single protein structure of sequence from Bacillus licheniformis. Full crystallographic information is available from OCA.
Reference
X-ray crystal structure of cross-linked subtilisin Carlsberg in water vs. acetonitrile., Fitzpatrick PA, Ringe D, Klibanov AM, Biochem Biophys Res Commun. 1994 Jan 28;198(2):675-81. PMID:8297378 Page seeded by OCA on Sat May 3 08:32:32 2008
