1sdj

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[[Image:1sdj.gif|left|200px]]
[[Image:1sdj.gif|left|200px]]
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{{Structure
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|PDB= 1sdj |SIZE=350|CAPTION= <scene name='initialview01'>1sdj</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_1sdj", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|GENE= YDDE, B1464 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK10281 PRK10281]</span>
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{{STRUCTURE_1sdj| PDB=1sdj | SCENE= }}
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|RELATEDENTRY=[[1p9v|1P9V]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sdj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sdj OCA], [http://www.ebi.ac.uk/pdbsum/1sdj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sdj RCSB]</span>
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'''X-RAY STRUCTURE OF YDDE_ECOLI NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ET25.'''
'''X-RAY STRUCTURE OF YDDE_ECOLI NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ET25.'''
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==About this Structure==
==About this Structure==
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1SDJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry 1P9V. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SDJ OCA].
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1SDJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1p9v 1p9v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SDJ OCA].
==Reference==
==Reference==
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[[Category: Skarina, T.]]
[[Category: Skarina, T.]]
[[Category: Tong, L.]]
[[Category: Tong, L.]]
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[[Category: nesg]]
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[[Category: Nesg]]
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[[Category: northeast structural genomics consortium]]
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[[Category: Northeast structural genomics consortium]]
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[[Category: protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: psi]]
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[[Category: Psi]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: unknown function]]
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[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:34:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:40:38 2008''
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Revision as of 05:34, 3 May 2008

Image:1sdj.gif

Template:STRUCTURE 1sdj

X-RAY STRUCTURE OF YDDE_ECOLI NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ET25.


Overview

Phenazines produced by Pseudomonas and Streptomyces spp. are heterocyclic nitrogen-containing metabolites with antibiotic, antitumor, and antiparasitic activity. The antibiotic properties of pyocyanin, produced by Pseudomonas aeruginosa, were recognized in the 1890s, although this blue phenazine is now known to be a virulence factor in human disease. Despite their biological significance, the biosynthesis of phenazines is not fully understood. Here we present structural and functional studies of PhzF, an enzyme essential for phenazine synthesis in Pseudomonas spp. PhzF shares topology with diaminopimelate epimerase DapF but lacks the same catalytic residues. The structure of PhzF in complex with its substrate, trans-2,3-dihydro-3-hydroxyanthranilic acid, suggests that it is an isomerase using the conserved glutamate E45 to abstract a proton from C3 of the substrate. The proton is returned to C1 of the substrate after rearrangement of the double-bond system, yielding an enol that converts to the corresponding ketone. PhzF is a dimer that may be bifunctional, providing a shielded cavity for ketone dimerization via double Schiff-base formation to produce the phenazine scaffold. Our proposed mechanism is supported by mass and NMR spectroscopy. The results are discussed in the context of related structures and protein sequences of unknown biochemical function.

About this Structure

1SDJ is a Single protein structure of sequence from Escherichia coli. This structure supersedes the now removed PDB entry 1p9v. Full crystallographic information is available from OCA.

Reference

Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens., Blankenfeldt W, Kuzin AP, Skarina T, Korniyenko Y, Tong L, Bayer P, Janning P, Thomashow LS, Mavrodi DV, Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16431-6. Epub 2004 Nov 15. PMID:15545603 Page seeded by OCA on Sat May 3 08:34:38 2008

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