1sdw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1sdw.gif|left|200px]]
[[Image:1sdw.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1sdw |SIZE=350|CAPTION= <scene name='initialview01'>1sdw</scene>, resolution 1.85&Aring;
+
The line below this paragraph, containing "STRUCTURE_1sdw", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IYT:N-ALPHA-ACETYL-3,5-DIIODOTYROSYL-D-THREONINE'>IYT</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidylglycine_monooxygenase Peptidylglycine monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.17.3 1.14.17.3] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= PAM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1sdw| PDB=1sdw | SCENE= }}
-
|RELATEDENTRY=[[1phm|1phm]], [[1opm|1opm]], [[3phm|3phm]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sdw OCA], [http://www.ebi.ac.uk/pdbsum/1sdw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sdw RCSB]</span>
+
-
}}
+
'''Reduced (Cu+) peptidylglycine alpha-hydroxylating monooxygenase with bound peptide and dioxygen'''
'''Reduced (Cu+) peptidylglycine alpha-hydroxylating monooxygenase with bound peptide and dioxygen'''
Line 30: Line 27:
[[Category: Mains, R E.]]
[[Category: Mains, R E.]]
[[Category: Prigge, S T.]]
[[Category: Prigge, S T.]]
-
[[Category: beta jelly-roll]]
+
[[Category: Beta jelly-roll]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:35:24 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:40:43 2008''
+

Revision as of 05:35, 3 May 2008

Image:1sdw.gif

Template:STRUCTURE 1sdw

Reduced (Cu+) peptidylglycine alpha-hydroxylating monooxygenase with bound peptide and dioxygen


Overview

Copper active sites play a major role in enzymatic activation of dioxygen. We trapped the copper-dioxygen complex in the enzyme peptidylglycine-alphahydroxylating monooxygenase (PHM) by freezing protein crystals that had been soaked with a slow substrate and ascorbate in the presence of oxygen. The x-ray crystal structure of this precatalytic complex, determined to 1.85-angstrom resolution, shows that oxygen binds to one of the coppers in the enzyme with an end-on geometry. Given this structure, it is likely that dioxygen is directly involved in the electron transfer and hydrogen abstraction steps of the PHM reaction. These insights may apply to other copper oxygen-activating enzymes, such as dopamine beta-monooxygenase, and to the design of biomimetic complexes.

About this Structure

1SDW is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Dioxygen binds end-on to mononuclear copper in a precatalytic enzyme complex., Prigge ST, Eipper BA, Mains RE, Amzel LM, Science. 2004 May 7;304(5672):864-7. PMID:15131304 Page seeded by OCA on Sat May 3 08:35:24 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sdw"
Personal tools