1sf8
From Proteopedia
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[[Image:1sf8.gif|left|200px]] | [[Image:1sf8.gif|left|200px]] | ||
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'''Crystal structure of the carboxy-terminal domain of htpG, the E. coli Hsp90''' | '''Crystal structure of the carboxy-terminal domain of htpG, the E. coli Hsp90''' | ||
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[[Category: Harris, S F.]] | [[Category: Harris, S F.]] | ||
[[Category: Shiau, A K.]] | [[Category: Shiau, A K.]] | ||
| - | [[Category: | + | [[Category: Exposed amphipathic helix]] |
| - | [[Category: | + | [[Category: Four helix bundle dimerization interface]] |
| - | [[Category: | + | [[Category: Three stranded beta sheet]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:37:44 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:37, 3 May 2008
Crystal structure of the carboxy-terminal domain of htpG, the E. coli Hsp90
Overview
Hsp90 is a ubiquitous, well-conserved molecular chaperone involved in the folding and stabilization of diverse proteins. Beyond its capacity for general protein folding, Hsp90 influences a wide array of cellular signaling pathways that underlie key biological and disease processes. It has been proposed that Hsp90 functions as a molecular clamp, dimerizing through its carboxy-terminal domain and utilizing ATP binding and hydrolysis to drive large conformational changes including transient dimerization of the amino-terminal and middle domains. We have determined the 2.6 A X-ray crystal structure of the carboxy-terminal domain of htpG, the Escherichia coli Hsp90. This structure reveals a novel fold and that dimerization is dependent upon the formation of a four-helix bundle. Remarkably, proximal to the helical dimerization motif, each monomer projects a short helix into solvent. The location, flexibility, and amphipathic character of this helix suggests that it may play a role in substrate binding and hence chaperone activity.
About this Structure
1SF8 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The crystal structure of the carboxy-terminal dimerization domain of htpG, the Escherichia coli Hsp90, reveals a potential substrate binding site., Harris SF, Shiau AK, Agard DA, Structure. 2004 Jun;12(6):1087-97. PMID:15274928 Page seeded by OCA on Sat May 3 08:37:44 2008
