1sg6

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[[Image:1sg6.gif|left|200px]]
[[Image:1sg6.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1sg6 |SIZE=350|CAPTION= <scene name='initialview01'>1sg6</scene>, resolution 1.7&Aring;
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The line below this paragraph, containing "STRUCTURE_1sg6", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-dehydroquinate_synthase 3-dehydroquinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.4 4.2.3.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= AROMA, AROM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=162425 Emericella nidulans])
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-->
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|DOMAIN=
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{{STRUCTURE_1sg6| PDB=1sg6 | SCENE= }}
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|RELATEDENTRY=[[1nrx|1NRX]], [[1nve|1NVE]], [[1nvd|1NVD]], [[1nvb|1NVB]], [[1nua|1NUA]], [[1nva|1NVA]], [[1nvf|1NVF]], [[1nr5|1NR5]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sg6 OCA], [http://www.ebi.ac.uk/pdbsum/1sg6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sg6 RCSB]</span>
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}}
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'''Crystal structure of Aspergillus nidulans 3-dehydroquinate synthase (AnDHQS) in complex with Zn2+ and NAD+, at 1.7D'''
'''Crystal structure of Aspergillus nidulans 3-dehydroquinate synthase (AnDHQS) in complex with Zn2+ and NAD+, at 1.7D'''
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[[Category: Nichols, C E.]]
[[Category: Nichols, C E.]]
[[Category: Stammers, D K.]]
[[Category: Stammers, D K.]]
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[[Category: aromatic amino acid biosynthesis]]
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[[Category: Aromatic amino acid biosynthesis]]
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[[Category: cyclase]]
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[[Category: Cyclase]]
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[[Category: dhq]]
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[[Category: Dhq]]
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[[Category: domain movement]]
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[[Category: Domain movement]]
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[[Category: form j]]
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[[Category: Form j]]
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[[Category: lyase]]
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[[Category: Lyase]]
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[[Category: open form]]
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[[Category: Open form]]
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[[Category: shikimate pathway]]
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[[Category: Shikimate pathway]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:39:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:41:36 2008''
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Revision as of 05:39, 3 May 2008

Image:1sg6.gif

Template:STRUCTURE 1sg6

Crystal structure of Aspergillus nidulans 3-dehydroquinate synthase (AnDHQS) in complex with Zn2+ and NAD+, at 1.7D


Overview

Crystallization of Aspergillus nidulans 3-dehydroquinate synthase (DHQS), following turnover of the enzyme by addition of the substrate DAHP, gave a new crystal form (form J). Although the crystals have dimensions of only 50 x 20 x 5 micro m, they are well ordered, diffracting to 1.7 A. The space group is C222(1), with unit-cell parameters a = 90.0, b = 103.7, c = 177.4 A. Structure determination and refinement to R = 0.19 (R(free) = 0.25) shows the DHQS is in the 'open' form with the substrate site unoccupied but with some loop regions perturbed. Previous crystals of open-form DHQS only diffracted to 2.5 A resolution. The use of enzyme turnover may be applicable in other systems in attempts to improve crystal quality.

About this Structure

1SG6 is a Single protein structure of sequence from Emericella nidulans. Full crystallographic information is available from OCA.

Reference

Structure of the 'open' form of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 A resolution from crystals grown following enzyme turnover., Nichols CE, Hawkins AR, Stammers DK, Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):971-3. Epub 2004, Apr 21. PMID:15103156 Page seeded by OCA on Sat May 3 08:39:46 2008

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