1sh4
From Proteopedia
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'''Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H''' | '''Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H''' | ||
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[[Category: Wu, H.]] | [[Category: Wu, H.]] | ||
[[Category: Zhang, Q.]] | [[Category: Zhang, Q.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:41:53 2008'' | |
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Revision as of 05:41, 3 May 2008
Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H
Overview
A comparative study on the solution structures of bovine microsomal cytochrome b5 (Tb5) and the mutant V45H has been achieved by 1D and 2D 1H-NMR spectroscopy to clarify the differences in the solution conformations between these two proteins. The results reveal that the global folding of the V45H mutant in solution is unchanged, but the subtle changes exist in the orientation of the axial ligand His39, and heme vinyl groups. The side chain of His45 in V45H mutant extends to the outer edge of the heme pocket leaving a cavity at the site originally occupied by the inner methyl group of Val45 residue. In addition, the imidazole ring of axial ligand His39 rotates counterclockwise by approximately 3 degrees around the His-Fe-His axis, and the 4-heme vinyl group turns to the space vacated by the removed side chain due to the mutation. Furthermore, the helix III of the heme pocket undergoes outward displacement, while the linkage between helix II and III is shifted leftward. These observations are not only consistent with the pattern of the pseudocontact shifts of the heme protons, but also well account for the lower stability of V45H mutant against heat and urea.
About this Structure
1SH4 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b5 and mutant V45H., Zhang Q, Cao C, Wang ZQ, Wang YH, Wu H, Huang ZX, Protein Sci. 2004 Aug;13(8):2161-9. PMID:15273310 Page seeded by OCA on Sat May 3 08:41:53 2008
Categories: Bos taurus | Single protein | Wu, H. | Zhang, Q. | Five helix | Five sheet | Heme ring
