1shk

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[[Image:1shk.gif|left|200px]]
[[Image:1shk.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1shk |SIZE=350|CAPTION= <scene name='initialview01'>1shk</scene>, resolution 1.9&Aring;
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The line below this paragraph, containing "STRUCTURE_1shk", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=PIP:A+Walker+Type+A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>PIP</scene>, <scene name='pdbsite=POP:A+Walker+Type+A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>POP</scene>, <scene name='pdbsite=SAS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SAS</scene> and <scene name='pdbsite=SBS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SBS</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= AROL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])
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-->
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|DOMAIN=
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{{STRUCTURE_1shk| PDB=1shk | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1shk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1shk OCA], [http://www.ebi.ac.uk/pdbsum/1shk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1shk RCSB]</span>
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}}
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'''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI'''
'''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI'''
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[[Category: Krell, T.]]
[[Category: Krell, T.]]
[[Category: Lapthorn, A J.]]
[[Category: Lapthorn, A J.]]
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[[Category: adp]]
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[[Category: Adp]]
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[[Category: p-loop protein]]
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[[Category: P-loop protein]]
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[[Category: phosphoryl transfer]]
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[[Category: Phosphoryl transfer]]
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[[Category: shikimate kinase]]
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[[Category: Shikimate kinase]]
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[[Category: shikimate pathway]]
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[[Category: Shikimate pathway]]
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[[Category: transferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:42:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:42:11 2008''
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Revision as of 05:42, 3 May 2008

Image:1shk.gif

Template:STRUCTURE 1shk

THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI


Overview

Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia coli has been crystallized by the vapour-diffusion method using sodium chloride as a precipitant. Mass spectrometry was used to confirm the purity of the shikimate kinase and dynamic light scattering was used to assess conditions for the monodispersity of the enzyme. The crystals are tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 3.6 A(3) Da(-1).

About this Structure

1SHK is a Single protein structure of sequence from Erwinia chrysanthemi. Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:15299895 Page seeded by OCA on Sat May 3 08:42:40 2008

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