|
|
| Line 1: |
Line 1: |
| | | | |
| | ==The solution structure of the REF2-I mRNA export factor (residues 1-155).== | | ==The solution structure of the REF2-I mRNA export factor (residues 1-155).== |
| - | <StructureSection load='2f3j' size='340' side='right'caption='[[2f3j]], [[NMR_Ensembles_of_Models | 14 NMR models]]' scene=''> | + | <StructureSection load='2f3j' size='340' side='right'caption='[[2f3j]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2f3j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F3J FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2f3j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F3J FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Refbp2, Ref2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f3j OCA], [https://pdbe.org/2f3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f3j RCSB], [https://www.ebi.ac.uk/pdbsum/2f3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f3j ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f3j OCA], [https://pdbe.org/2f3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f3j RCSB], [https://www.ebi.ac.uk/pdbsum/2f3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f3j ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ALRF2_MOUSE ALRF2_MOUSE]] Export adapter involved in spliced and unspliced mRNA nuclear export. Binds mRNA which is transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway); enhances NXF1-NXT1 RNA-binding activity.<ref>PMID:10786854</ref> <ref>PMID:11158589</ref> <ref>PMID:18364396</ref>
| + | [https://www.uniprot.org/uniprot/ALRF2_MOUSE ALRF2_MOUSE] Export adapter involved in spliced and unspliced mRNA nuclear export. Binds mRNA which is transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway); enhances NXF1-NXT1 RNA-binding activity.<ref>PMID:10786854</ref> <ref>PMID:11158589</ref> <ref>PMID:18364396</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 33: |
Line 33: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Golovanov, A P]] | + | [[Category: Golovanov AP]] |
| - | [[Category: Hautbergue, G M]] | + | [[Category: Hautbergue GM]] |
| - | [[Category: Lian, L Y]] | + | [[Category: Lian LY]] |
| - | [[Category: Wilson, S A]] | + | [[Category: Wilson SA]] |
| - | [[Category: Rbd domain]]
| + | |
| - | [[Category: Rrm domain]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
ALRF2_MOUSE Export adapter involved in spliced and unspliced mRNA nuclear export. Binds mRNA which is transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway); enhances NXF1-NXT1 RNA-binding activity.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The RNA binding and export factor (REF) family of mRNA export adaptors are found in several nuclear protein complexes including the spliceosome, TREX, and exon junction complexes. They bind RNA, interact with the helicase UAP56/DDX39, and are thought to bridge the interaction between the export factor TAP/NXF1 and mRNA. REF2-I consists of three domains, with the RNA recognition motif (RRM) domain positioned in the middle. Here we dissect the interdomain interactions of REF2-I and present the solution structure of a functionally competent double domain (NM; residues 1-155). The N-terminal domain comprises a transient helix (N-helix) linked to the RRM by a flexible arm that includes an Arg-rich region. The N-helix, which is required for REF2-I function in vivo, overlaps the highly conserved REF-N motif and, together with the adjacent Arg-rich region, interacts transiently with the RRM. RNA interacts with REF2-I through arginine-rich regions in its N- and C-terminal domains, but we show that it also interacts weakly with the RRM. The mode of interaction is unusual for an RRM since it involves loops L1 and L5. NMR signal mapping and biochemical analysis with NM indicate that DDX39 and TAP interact with both the N and RRM domains of REF2-I and show that binding of these proteins and RNA will favor an open conformation for the two domains. The proximity of the RNA, TAP, and DDX39 binding sites on REF2-I suggests their binding may be mutually exclusive, which would lead to successive ligand binding events in the course of mRNA export.
The solution structure of REF2-I reveals interdomain interactions and regions involved in binding mRNA export factors and RNA.,Golovanov AP, Hautbergue GM, Tintaru AM, Lian LY, Wilson SA RNA. 2006 Nov;12(11):1933-48. Epub 2006 Sep 25. PMID:17000901[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Stutz F, Bachi A, Doerks T, Braun IC, Seraphin B, Wilm M, Bork P, Izaurralde E. REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export. RNA. 2000 Apr;6(4):638-50. PMID:10786854
- ↑ Rodrigues JP, Rode M, Gatfield D, Blencowe BJ, Carmo-Fonseca M, Izaurralde E. REF proteins mediate the export of spliced and unspliced mRNAs from the nucleus. Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1030-5. Epub 2001 Jan 23. PMID:11158589 doi:http://dx.doi.org/10.1073/pnas.031586198
- ↑ Hautbergue GM, Hung ML, Golovanov AP, Lian LY, Wilson SA. Mutually exclusive interactions drive handover of mRNA from export adaptors to TAP. Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5154-9. doi:, 10.1073/pnas.0709167105. Epub 2008 Mar 25. PMID:18364396 doi:http://dx.doi.org/10.1073/pnas.0709167105
- ↑ Golovanov AP, Hautbergue GM, Tintaru AM, Lian LY, Wilson SA. The solution structure of REF2-I reveals interdomain interactions and regions involved in binding mRNA export factors and RNA. RNA. 2006 Nov;12(11):1933-48. Epub 2006 Sep 25. PMID:17000901 doi:10.1261/rna.212106
|
