1siy
From Proteopedia
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'''NMR structure of mung bean non-specific lipid transfer protein 1''' | '''NMR structure of mung bean non-specific lipid transfer protein 1''' | ||
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[[Category: Lyu, P C.]] | [[Category: Lyu, P C.]] | ||
[[Category: Samuel, D.]] | [[Category: Samuel, D.]] | ||
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Revision as of 05:45, 3 May 2008
NMR structure of mung bean non-specific lipid transfer protein 1
Overview
Plant nonspecific lipid transfer proteins (nsLTPs) are thermal stable proteins that are capable of transferring lipid molecules between bilayers in vitro. This family of proteins, abundant in plants, is proposed to be involved in defense, pollination, and germination; the in vivo biological function remains, however, elusive. Here we report the purification and sequencing of an nsLTP1 from mung bean sprouts. We have also determined the solution structure of this nsLTP1, which represents the first 3D structure of the dicotyledonous nsLTP1 family. The global fold of mung bean nsLTP1 is similar to those of the monocotyledonous nsLTP1 structures and consists of four alpha-helices stabilized by four disulfide bonds. There are, however, some notable differences in the C-terminal tails and internal hydrophobic cavities. Circular dichroism and fluorescence spectroscopy were used to compare the thermodynamics and lipid transfer properties of mung bean nsLTP1 with those of rice nsLTP1. Docking of a lipid molecule into the solution structure of mung bean nsLTP1 reveals similar binding cavities and hydrophobic interactions as in rice nsLTP1, consistent with their comparable lipid transfer properties measured experimentally.
About this Structure
1SIY is a Single protein structure of sequence from Vigna radiata var. radiata. Full crystallographic information is available from OCA.
Reference
Characterization and structural analyses of nonspecific lipid transfer protein 1 from mung bean., Lin KF, Liu YN, Hsu ST, Samuel D, Cheng CS, Bonvin AM, Lyu PC, Biochemistry. 2005 Apr 19;44(15):5703-12. PMID:15823028 Page seeded by OCA on Sat May 3 08:45:26 2008
