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| | ==Solution structure of Bem1p PB1 domain complexed with Cdc24p PB1 domain== | | ==Solution structure of Bem1p PB1 domain complexed with Cdc24p PB1 domain== |
| - | <StructureSection load='2kfk' size='340' side='right'caption='[[2kfk]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2kfk' size='340' side='right'caption='[[2kfk]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2kfk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2kfk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFK FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfk OCA], [https://pdbe.org/2kfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfk RCSB], [https://www.ebi.ac.uk/pdbsum/2kfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfk ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfk OCA], [https://pdbe.org/2kfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfk RCSB], [https://www.ebi.ac.uk/pdbsum/2kfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/BEM1_YEAST BEM1_YEAST]] Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface.<ref>PMID:17460121</ref> [[https://www.uniprot.org/uniprot/CDC24_YEAST CDC24_YEAST]] Promotes the exchange of CDC42-bound GDP by GTP. Controls the polarity of calmodulin, and the calcium regulatory process of bud emergence. CDC24 may be involved in the initial selection and organization of the budding site.
| + | [https://www.uniprot.org/uniprot/BEM1_YEAST BEM1_YEAST] Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface.<ref>PMID:17460121</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Inagaki, F]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Kobashigawa, Y]] | + | [[Category: Inagaki F]] |
| - | [[Category: Ogura, K]] | + | [[Category: Kobashigawa Y]] |
| - | [[Category: Tandai, T]] | + | [[Category: Ogura K]] |
| - | [[Category: Yoshinaga, S]] | + | [[Category: Tandai T]] |
| - | [[Category: Budding]]
| + | [[Category: Yoshinaga S]] |
| - | [[Category: Pb1]]
| + | |
| - | [[Category: Phox]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Yeast]]
| + | |
| Structural highlights
Function
BEM1_YEAST Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Bem1 and Cdc24 of the budding yeast Saccharomyces cerevisiae interact with each other through PB1-PB1 heterodimer formation to regulate the establishment of cell polarity. Here we present the tertiary structure of the heterodimer of Bem1 and Cdc24 PB1 domains determined by NMR spectroscopy. To avoid ambiguity in the NMR spectral analysis, we first prepared a mutant of the Cdc24 PB1 domain that had truncated loops. The mutant provided well dispersed spectra without spectral overlapping, thus allowing unambiguous spectral assignments for structure determination. We confirmed that the loop deletion-mutant was quite similar to the wild-type in both 3D structure and binding affinity. The NMR structure of the heterodimer of the deletion-mutant of Cdc24 PB1 and Bem1 PB1 was determined using a variety of isotope labelled samples including perdeuteration. The interface between the Bem1/Cdc24 PB1 heterodimer was analysed at atomic resolution. Through a comparison with the tertiary structures of other PB1-PB1 heterodimers, we found that conserved electrostatic properties on the molecular surface were commonly used for PB1-PB1 interaction, but hydrophobic interactions were important for cognate interaction in Bem1/Cdc24 PB1 heterodimer formation.
NMR structure of the heterodimer of Bem1 and Cdc24 PB1 domains from Saccharomyces cerevisiae.,Ogura K, Tandai T, Yoshinaga S, Kobashigawa Y, Kumeta H, Ito T, Sumimoto H, Inagaki F J Biochem. 2009 Sep;146(3):317-25. Epub 2009 May 18. PMID:19451149[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gao XD, Sperber LM, Kane SA, Tong Z, Tong AH, Boone C, Bi E. Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle. Mol Biol Cell. 2007 Jul;18(7):2542-60. Epub 2007 Apr 25. PMID:17460121 doi:http://dx.doi.org/10.1091/mbc.E06-09-0822
- ↑ Ogura K, Tandai T, Yoshinaga S, Kobashigawa Y, Kumeta H, Ito T, Sumimoto H, Inagaki F. NMR structure of the heterodimer of Bem1 and Cdc24 PB1 domains from Saccharomyces cerevisiae. J Biochem. 2009 Sep;146(3):317-25. Epub 2009 May 18. PMID:19451149 doi:10.1093/jb/mvp075
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