1w1f
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(New page: 200px<br /> <applet load="1w1f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w1f" /> '''SH3 DOMAIN OF HUMAN LYN TYROSINE KINASE'''<...)
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Revision as of 17:39, 12 November 2007
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SH3 DOMAIN OF HUMAN LYN TYROSINE KINASE
Overview
The Src homology 3 (SH3) domain of the Src family kinase Lyn binds to the, herpesviral tyrosine kinase interacting protein (Tip) more than one order, of magnitude stronger than other closely related members of the Src, family. In order to identify the molecular basis for high-affinity, binding, the structure of free and Tip-bound Lyn-SH3 was determined by NMR, spectroscopy. Tip forms additional contacts outside its classical, proline-rich recognition motif and, in particular, a strictly conserved, leucine (L186) of the C-terminally adjacent sequence stretch packs into a, hydrophobic pocket on the Lyn surface. Although the existence of this, pocket is no unique property of Lyn-SH3, Lyn is the only Src family kinase, that contains an additional aromatic residue (H41) in the n-Src loop as, part of this pocket. H41 covers L186 of Tip by forming tight hydrophobic, contacts, and model calculations suggest that the increase in binding, affinity compared with other SH3 domains can mainly be attributed to these, additional interactions. These findings indicate that this pocket can, mediate specificity even between otherwise closely related SH3 domains.
About this Structure
1W1F is a Single protein structure of sequence from Homo sapiens. Active as Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 Full crystallographic information is available from OCA.
Reference
Structural characterization of Lyn-SH3 domain in complex with a herpesviral protein reveals an extended recognition motif that enhances binding affinity., Bauer F, Schweimer K, Meiselbach H, Hoffmann S, Rosch P, Sticht H, Protein Sci. 2005 Oct;14(10):2487-98. Epub 2005 Sep 9. PMID:16155203
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