4q2m
From Proteopedia
(Difference between revisions)
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==Structure of the E. coli YajR Transporter YAM Domain Combined Iodine== | ==Structure of the E. coli YajR Transporter YAM Domain Combined Iodine== | ||
| - | <StructureSection load='4q2m' size='340' side='right'caption='[[4q2m]]' scene=''> | + | <StructureSection load='4q2m' size='340' side='right'caption='[[4q2m]], [[Resolution|resolution]] 1.49Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q2M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4q2m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q2M FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2m OCA], [https://pdbe.org/4q2m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q2m RCSB], [https://www.ebi.ac.uk/pdbsum/4q2m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2m ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.489Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2m OCA], [https://pdbe.org/4q2m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q2m RCSB], [https://www.ebi.ac.uk/pdbsum/4q2m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2m ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | YajR is an Escherichia coli transporter that belongs to the major facilitator superfamily. Unlike most MFS transporters, YajR contains a carboxyl terminal, cytosolic domain of 67 amino acid residues termed YAM domain. Although it is speculated that the function of this small soluble domain is to regulate the conformational change of the 12-helix transmembrane domain, its precise regulatory role remains unclear. Here, we report the crystal structure of the YAM domain at 1.07-A resolution, along with its structure determined using nuclear magnetic resonance. Detailed analysis of the high resolution structure revealed a symmetrical dimer in which a belt of well-ordered poly-pentagonal water molecules is embedded. A mutagenesis experiment and a thermal stability assay were used to analyze the putative role of this dimerization in response to changes in halogen concentration. | ||
| + | |||
| + | Atomic resolution structure of the E. coli YajR transporter YAM domain.,Jiang D, Zhao Y, Fan J, Liu X, Wu Y, Feng W, Zhang XC Biochem Biophys Res Commun. 2014 Jun 19. pii: S0006-291X(14)01127-9. doi:, 10.1016/j.bbrc.2014.06.053. PMID:24952155<ref>PMID:24952155</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4q2m" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Zhang XC]] | [[Category: Zhang XC]] | ||
Current revision
Structure of the E. coli YajR Transporter YAM Domain Combined Iodine
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