1sjj
From Proteopedia
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'''Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin''' | '''Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin''' | ||
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[[Category: Taylor, K A.]] | [[Category: Taylor, K A.]] | ||
[[Category: 3-helix bundle]] | [[Category: 3-helix bundle]] | ||
| - | [[Category: | + | [[Category: Actin binding protein]] |
| - | [[Category: | + | [[Category: Calmodulin-like domain]] |
| - | [[Category: | + | [[Category: Calponin homology domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:46:55 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:46, 3 May 2008
Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin
Overview
Cryoelectron microscopy was used to obtain a 3-D image at 2.0 nm resolution of 2-D arrays of smooth muscle alpha-actinin. The reconstruction reveals a well-resolved long central domain with 90 degrees of left-handed twist and near 2-fold symmetry. However, the molecular ends which contain the actin binding and calmodulin-like domains, have different structures oriented approximately 90 degrees to each other. Atomic structures for the alpha-actinin domains were built by homology modeling and assembled into an atomic model. Model building suggests that in the 2-D arrays, the two calponin homology domains that comprise the actin-binding domain have a closed conformation at one end and an open conformation at the other end due to domain swapping. The open and closed conformations of the actin-binding domain suggests flexibility that may underlie Ca2+ regulation. The approximately 90 degrees orientation difference at the molecular ends may underlie alpha-actinin's ability to crosslink actin filaments in nearly any orientation.
About this Structure
1SJJ is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
A 3-D reconstruction of smooth muscle alpha-actinin by CryoEm reveals two different conformations at the actin-binding region., Liu J, Taylor DW, Taylor KA, J Mol Biol. 2004 Apr 16;338(1):115-25. PMID:15050827 Page seeded by OCA on Sat May 3 08:46:55 2008
