6jb3

From Proteopedia

(Difference between revisions)

Current revision

Structure of SUR1 subunit bound with repaglinide

6jb3, resolution 3.53Å

Structural highlights

6jb3 is a 1 chain structure with sequence from Mesocricetus auratus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.53Å
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A1U7R319_MESAU

Publication Abstract from PubMed

Repaglinide (RPG) is a short-acting insulin secretagogue widely prescribed for the treatment of type 2 diabetes. It boosts insulin secretion by inhibiting the pancreatic ATP-sensitive potassium channel (KATP). However, the mechanisms by which RPG binds to the KATP channel are poorly understood. Here, we describe two cryo-EM structures: the pancreatic KATP channel in complex with inhibitory RPG and adenosine-5'-(gamma-thio)-triphosphate (ATPgammaS) at 3.3 A and a medium-resolution structure of a RPG-bound mini SUR1 protein in which the N terminus of the inward-rectifying potassium channel 6.1 (Kir6.1) is fused to the ABC transporter module of the sulfonylurea receptor 1 (SUR1). These structures reveal the binding site of RPG in the SUR1 subunit. Furthermore, the high-resolution structure reveals the complex architecture of the ATP binding site, which is formed by both Kir6.2 and SUR1 subunits, and the domain-domain interaction interfaces.

The Structural Basis for the Binding of Repaglinide to the Pancreatic KATP Channel.,Ding D, Wang M, Wu JX, Kang Y, Chen L Cell Rep. 2019 May 7;27(6):1848-1857.e4. doi: 10.1016/j.celrep.2019.04.050. PMID:31067468^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ding D, Wang M, Wu JX, Kang Y, Chen L. The Structural Basis for the Binding of Repaglinide to the Pancreatic KATP Channel. Cell Rep. 2019 May 7;27(6):1848-1857.e4. doi: 10.1016/j.celrep.2019.04.050. PMID:31067468 doi:http://dx.doi.org/10.1016/j.celrep.2019.04.050

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6jb3"

@@ Line 1: / Line 1: @@
 ==Structure of SUR1 subunit bound with repaglinide==
-<SX load='6jb3' size='340' side='right' viewer='molstar' caption='[[6jb3]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+<SX load='6jb3' size='340' side='right' viewer='molstar' caption='[[6jb3]], [[Resolution|resolution]] 3.53&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6jb3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Golden_hamster Golden hamster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JB3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JB3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6jb3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesocricetus_auratus Mesocricetus auratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JB3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JB3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AJP:Digitonin'>AJP</scene>, <scene name='pdbligand=BJX:Repaglinide'>BJX</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.53&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Abcc8 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10036 Golden hamster])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AJP:Digitonin'>AJP</scene>, <scene name='pdbligand=BJX:Repaglinide'>BJX</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jb3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jb3 OCA], [https://pdbe.org/6jb3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jb3 RCSB], [https://www.ebi.ac.uk/pdbsum/6jb3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jb3 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A1U7R319_MESAU A0A1U7R319_MESAU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </SX>
-[[Category: Golden hamster]]
 [[Category: Large Structures]]
-[[Category: Chen, L]]
+[[Category: Mesocricetus auratus]]
-[[Category: Ding, D]]
+[[Category: Chen L]]
-[[Category: Kang, Y]]
+[[Category: Ding D]]
-[[Category: Wang, M]]
+[[Category: Kang Y]]
-[[Category: Wu, J X]]
+[[Category: Wang M]]
-[[Category: Abc transporter]]
+[[Category: Wu J-X]]
-[[Category: Channel]]
-[[Category: Diabetes]]
-[[Category: Insulin secretagogue]]
-[[Category: Katp]]
-[[Category: Kir]]
-[[Category: Membrane protein]]
-[[Category: Repaglinide]]
-[[Category: Sur]]

6jb3

From Proteopedia

Current revision

Structure of SUR1 subunit bound with repaglinide

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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