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6m6r
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Crystal structure of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3) C-terminal domain with 5'-ppp 8-mer ssRNA== |
| - | <StructureSection load='6m6r' size='340' side='right'caption='[[6m6r]]' scene=''> | + | <StructureSection load='6m6r' size='340' side='right'caption='[[6m6r]], [[Resolution|resolution]] 1.89Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6m6r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M6R FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m6r OCA], [https://pdbe.org/6m6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m6r RCSB], [https://www.ebi.ac.uk/pdbsum/6m6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m6r ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m6r OCA], [https://pdbe.org/6m6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m6r RCSB], [https://www.ebi.ac.uk/pdbsum/6m6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m6r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q93413_CAEEL Q93413_CAEEL] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DRH-3 is critically involved in germline development and RNA interference (RNAi) facilitated chromosome segregation via the 22G-siRNA pathway in Caenorhabditis elegans. DRH-3 has similar domain architecture to RIG-I-like receptors (RLRs) and belongs to the RIG-I-like RNA helicase family. The molecular understanding of DRH-3 and its function in endogenous RNAi pathways remains elusive. In this study, we solved the crystal structures of the DRH-3 N-terminal domain (NTD) and the C-terminal domains (CTDs) in complex with 5'-triphosphorylated RNAs. The NTD of DRH-3 adopts a distinct fold of tandem caspase activation and recruitment domains (CARDs) structurally similar to the CARDs of RIG-I and MDA5, suggesting a signaling function in the endogenous RNAi biogenesis. The CTD preferentially recognizes 5'-triphosphorylated double-stranded RNAs bearing the typical features of secondary siRNA transcripts. The full-length DRH-3 displays unique structural dynamics upon binding to RNA duplexes that differ from RIG-I or MDA5. These features of DRH-3 showcase the evolutionary divergence of the Dicer and RLR family of helicases. | ||
| + | |||
| + | Insights into the structure and RNA-binding specificity of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3).,Li K, Zheng J, Wirawan M, Trinh NM, Fedorova O, Griffin PR, Pyle AM, Luo D Nucleic Acids Res. 2021 Sep 27;49(17):9978-9991. doi: 10.1093/nar/gkab712. PMID:34403472<ref>PMID:34403472</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6m6r" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Caenorhabditis elegans]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Z | + | [[Category: Synthetic construct]] |
| + | [[Category: Fedorova O]] | ||
| + | [[Category: Griffin P]] | ||
| + | [[Category: Li K]] | ||
| + | [[Category: Luo D]] | ||
| + | [[Category: Plyle A]] | ||
| + | [[Category: Wirawan M]] | ||
| + | [[Category: Xiong Z]] | ||
| + | [[Category: Zheng J]] | ||
Current revision
Crystal structure of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3) C-terminal domain with 5'-ppp 8-mer ssRNA
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Categories: Caenorhabditis elegans | Large Structures | Synthetic construct | Fedorova O | Griffin P | Li K | Luo D | Plyle A | Wirawan M | Xiong Z | Zheng J
