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| | <SX load='6v20' size='340' side='right' viewer='molstar' caption='[[6v20]], [[Resolution|resolution]] 2.13Å' scene=''> | | <SX load='6v20' size='340' side='right' viewer='molstar' caption='[[6v20]], [[Resolution|resolution]] 2.13Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6v20]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V20 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6v20]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V20 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ALDOA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.13Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v20 OCA], [https://pdbe.org/6v20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v20 RCSB], [https://www.ebi.ac.uk/pdbsum/6v20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v20 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v20 OCA], [https://pdbe.org/6v20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v20 RCSB], [https://www.ebi.ac.uk/pdbsum/6v20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v20 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ALDOA_RABIT ALDOA_RABIT]] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.<ref>PMID:17329259</ref>
| + | [https://www.uniprot.org/uniprot/ALDOA_RABIT ALDOA_RABIT] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.<ref>PMID:17329259</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: European rabbit]] | |
| - | [[Category: Fructose-bisphosphate aldolase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Herzik, M A]] | + | [[Category: Oryctolagus cuniculus]] |
| - | [[Category: Lander, G C]] | + | [[Category: Herzik MA]] |
| - | [[Category: Wu, M]] | + | [[Category: Lander GC]] |
| - | [[Category: D-fructose]] | + | [[Category: Wu M]] |
| - | [[Category: Glycolysis]]
| + | |
| - | [[Category: Homo-4-mer]]
| + | |
| - | [[Category: Lyase]]
| + | |
| Structural highlights
Function
ALDOA_RABIT Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.[1]
Publication Abstract from PubMed
Although the advent of direct electron detectors (DEDs) and software developments have enabled the routine use of single-particle cryogenic electron microscopy (cryo-EM) for structure determination of well-behaved specimens to high-resolution, there nonetheless remains a discrepancy between the resolutions attained for biological specimens and the information limits of modern transmission electron microscopes (TEMs). Instruments operating at 300kV equipped with DEDs are the current paradigm for high-resolution single-particle cryo-EM, while 200kV TEMs remain comparatively underutilized for purposes beyond sample screening. Here, we expand upon our prior work and demonstrate that one such 200kV microscope, the Talos Arctica, equipped with a K2 DED is capable of determining structures of macromolecules to as high as approximately 1.7A resolution. At this resolution, ordered water molecules are readily assigned and holes in aromatic residues can be clearly distinguished in the reconstructions. This work emphasizes the utility of 200kV electrons for high-resolution single-particle cryo-EM and applications such as structure-based drug design.
Sub-2 Angstrom resolution structure determination using single-particle cryo-EM at 200keV.,Wu M, Lander GC, Herzik MA Jr J Struct Biol X. 2020 Feb 28;4:100020. doi: 10.1016/j.yjsbx.2020.100020., eCollection 2020. PMID:32647824[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ St-Jean M, Izard T, Sygusch J. A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome protein. J Biol Chem. 2007 May 11;282(19):14309-15. Epub 2007 Feb 27. PMID:17329259 doi:10.1074/jbc.M611505200
- ↑ Wu M, Lander GC, Herzik MA Jr. Sub-2 Angstrom resolution structure determination using single-particle cryo-EM at 200keV. J Struct Biol X. 2020 Feb 28;4:100020. doi: 10.1016/j.yjsbx.2020.100020., eCollection 2020. PMID:32647824 doi:http://dx.doi.org/10.1016/j.yjsbx.2020.100020
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