7f7p
From Proteopedia
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==AcrIIC4== | ==AcrIIC4== | ||
| - | <StructureSection load='7f7p' size='340' side='right'caption='[[7f7p]]' scene=''> | + | <StructureSection load='7f7p' size='340' side='right'caption='[[7f7p]], [[Resolution|resolution]] 2.03Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F7P FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F7P FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f7p OCA], [https://pdbe.org/7f7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f7p RCSB], [https://www.ebi.ac.uk/pdbsum/7f7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f7p ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f7p OCA], [https://pdbe.org/7f7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f7p RCSB], [https://www.ebi.ac.uk/pdbsum/7f7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f7p ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Clustered regularly interspaced short palindromic repeats (CRISPRs)-CRISPR-associated protein systems are bacterial and archaeal defense mechanisms against invading elements such as phages and viruses. To overcome these defense systems, phages and viruses have developed inhibitors called anti-CRISPRs (Acrs) that are capable of inhibiting the host CRISPR-Cas system via different mechanisms. Although the inhibitory mechanisms of AcrIIC1, AcrIIC2, and AcrIIC3 have been revealed, the inhibitory mechanisms of AcrIIC4 and AcrIIC5 have not been fully understood and structural data are unavailable. In this study, we elucidated the crystal structure of Type IIC anti-CRISPR protein, AcrIIC4. Our structural analysis revealed that AcrIIC4 exhibited a helical bundle fold comprising four helixes. Further biochemical and biophysical analyses showed that AcrIIC4 formed a monomer in solution, and monomeric AcrIIC4 directly interacted with Cas9 and Cas9/sgRNA complex. Discovery of the structure of AcrIIC4 and their interaction mode on Cas9 will help us elucidate the diversity in the inhibitory mechanisms of the Acr protein family. | ||
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| + | Crystal structure of the anti-CRISPR, AcrIIC4.,Kim GE, Lee SY, Park HH Protein Sci. 2021 Dec;30(12):2474-2481. doi: 10.1002/pro.4214. Epub 2021 Oct 29. PMID:34676610<ref>PMID:34676610</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7f7p" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
AcrIIC4
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