7jsn
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Structure of the Visual Signaling Complex between Transducin and Phosphodiesterase 6== |
| - | <StructureSection load='7jsn' size='340' side='right'caption='[[7jsn]]' scene=''> | + | <StructureSection load='7jsn' size='340' side='right'caption='[[7jsn]], [[Resolution|resolution]] 3.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7jsn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JSN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JSN FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jsn OCA], [https://pdbe.org/7jsn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jsn RCSB], [https://www.ebi.ac.uk/pdbsum/7jsn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jsn ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=35G:GUANOSINE-3,5-MONOPHOSPHATE'>35G</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VDN:2-{2-ETHOXY-5-[(4-ETHYLPIPERAZIN-1-YL)SULFONYL]PHENYL}-5-METHYL-7-PROPYLIMIDAZO[5,1-F][1,2,4]TRIAZIN-4(1H)-ONE'>VDN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jsn OCA], [https://pdbe.org/7jsn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jsn RCSB], [https://www.ebi.ac.uk/pdbsum/7jsn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jsn ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PDE6A_BOVIN PDE6A_BOVIN] This protein participates in processes of transmission and amplification of the visual signal. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Heterotrimeric G proteins communicate signals from activated G protein-coupled receptors to downstream effector proteins. In the phototransduction pathway responsible for vertebrate vision, the G protein-effector complex is composed of the GTP-bound transducin alpha subunit (GalphaT.GTP) and the cyclic GMP (cGMP) phosphodiesterase 6 (PDE6), which stimulates cGMP hydrolysis, leading to hyperpolarization of the photoreceptor cell. Here we report a cryo-electron microscopy (cryoEM) structure of PDE6 complexed to GTP-bound GalphaT. The structure reveals two GalphaT.GTP subunits engaging the PDE6 hetero-tetramer at both the PDE6 catalytic core and the PDEgamma subunits, driving extensive rearrangements to relieve all inhibitory constraints on enzyme catalysis. Analysis of the conformational ensemble in the cryoEM data highlights the dynamic nature of the contacts between the two GalphaT.GTP subunits and PDE6 that supports an alternating-site catalytic mechanism. | ||
| + | |||
| + | Structure of the Visual Signaling Complex between Transducin and Phosphodiesterase 6.,Gao Y, Eskici G, Ramachandran S, Poitevin F, Seven AB, Panova O, Skiniotis G, Cerione RA Mol Cell. 2020 Oct 15;80(2):237-245.e4. doi: 10.1016/j.molcel.2020.09.013. Epub, 2020 Oct 1. PMID:33007200<ref>PMID:33007200</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7jsn" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Phosphodiesterase 3D structures|Phosphodiesterase 3D structures]] | ||
| + | *[[Transducin 3D structures|Transducin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bos taurus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Cerione RA]] |
| + | [[Category: Eskici G]] | ||
| + | [[Category: Gao Y]] | ||
| + | [[Category: Ramachandran S]] | ||
| + | [[Category: Skiniotis G]] | ||
Current revision
Structure of the Visual Signaling Complex between Transducin and Phosphodiesterase 6
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