7lb6
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==PDX1.2/PDX1.3 co-expression complex== |
| - | <StructureSection load='7lb6' size='340' side='right'caption='[[7lb6]]' scene=''> | + | <StructureSection load='7lb6' size='340' side='right'caption='[[7lb6]], [[Resolution|resolution]] 3.16Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7lb6]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LB6 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lb6 OCA], [https://pdbe.org/7lb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lb6 RCSB], [https://www.ebi.ac.uk/pdbsum/7lb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lb6 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.16Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lb6 OCA], [https://pdbe.org/7lb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lb6 RCSB], [https://www.ebi.ac.uk/pdbsum/7lb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lb6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PDX12_ARATH PDX12_ARATH] The protein has no function in the formation of pyridoxal 5'-phosphate.<ref>PMID:17468224</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pseudoenzymes have emerged as key regulatory elements in all kingdoms of life despite being catalytically nonactive. Yet many factors defining why one protein is active while its homologue is inactive remain uncertain. For pseudoenzyme-enzyme pairs, the similarity of both subunits can often hinder conventional characterization approaches. In plants, a pseudoenzyme, PDX1.2, positively regulates vitamin B6 production by association with its active catalytic homologues such as PDX1.3 through an unknown assembly mechanism. Here we used an integrative experimental approach to learn that such pseudoenzyme-enzyme pair associations result in heterocomplexes of variable stoichiometry, which are unexpectedly tunable. We also present the atomic structure of the PDX1.2 pseudoenzyme as well as the population averaged PDX1.2-PDX1.3 pseudoenzyme-enzyme pair. Finally, we dissected hetero-dodecamers of each stoichiometry to understand the arrangement of monomers in the heterocomplexes and identified symmetry-imposed preferences in PDX1.2-PDX1.3 interactions. Our results provide a new model of pseudoenzyme-enzyme interactions and their native heterogeneity. | ||
| + | |||
| + | Tunable Heteroassembly of a Plant Pseudoenzyme-Enzyme Complex.,Novikova IV, Zhou M, Du C, Parra M, Kim DN, VanAernum ZL, Shaw JB, Hellmann H, Wysocki VH, Evans JE ACS Chem Biol. 2021 Sep 14. doi: 10.1021/acschembio.1c00475. PMID:34520180<ref>PMID:34520180</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7lb6" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Arabidopsis thaliana]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Evans JE]] |
| + | [[Category: Novikova IV]] | ||
Current revision
PDX1.2/PDX1.3 co-expression complex
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