7n0k
From Proteopedia
(Difference between revisions)
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==Cryo-EM structure of TACAN in the apo form (TMEM120A)== | ==Cryo-EM structure of TACAN in the apo form (TMEM120A)== | ||
| - | <StructureSection load='7n0k' size='340' side='right'caption='[[7n0k]]' scene=''> | + | <StructureSection load='7n0k' size='340' side='right'caption='[[7n0k]], [[Resolution|resolution]] 3.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N0K FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N0K FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n0k OCA], [https://pdbe.org/7n0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n0k RCSB], [https://www.ebi.ac.uk/pdbsum/7n0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n0k ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n0k OCA], [https://pdbe.org/7n0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n0k RCSB], [https://www.ebi.ac.uk/pdbsum/7n0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n0k ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mechanosensitive ion channels mediate transmembrane ion currents activated by mechanical forces. A mechanosensitive ion channel called TACAN was recently reported. We began to study TACAN with the intent to understand how it senses mechanical forces and functions as an ion channel. Using cellular patch-recording methods, we failed to identify mechanosensitive ion channel activity. Using membrane reconstitution methods, we found that TACAN, at high protein concentrations, produces heterogeneous conduction levels that are not mechanosensitive and are most consistent with disruptions of the lipid bilayer. We determined the structure of TACAN using single-particle cryo-electron microscopy and observed that it is a symmetrical dimeric transmembrane protein. Each protomer contains an intracellular-facing cleft with a coenzyme A cofactor, confirmed by mass spectrometry. The TACAN protomer is related in three-dimensional structure to a fatty acid elongase, ELOVL7. Whilst its physiological function remains unclear, we anticipate that TACAN is not a mechanosensitive ion channel. | ||
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| + | Analysis of the mechanosensor channel functionality of TACAN.,Niu Y, Tao X, Vaisey G, Olinares PDB, Alwaseem H, Chait BT, MacKinnon R Elife. 2021 Aug 10;10. pii: 71188. doi: 10.7554/eLife.71188. PMID:34374644<ref>PMID:34374644</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7n0k" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Cryo-EM structure of TACAN in the apo form (TMEM120A)
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