7n3p
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Cryo-EM structure of the Cas12k-sgRNA-dsDNA complex== |
| - | <StructureSection load='7n3p' size='340' side='right'caption='[[7n3p]]' scene=''> | + | <StructureSection load='7n3p' size='340' side='right'caption='[[7n3p]], [[Resolution|resolution]] 3.65Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7n3p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Scytonema_hofmannii Scytonema hofmannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N3P FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n3p OCA], [https://pdbe.org/7n3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n3p RCSB], [https://www.ebi.ac.uk/pdbsum/7n3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n3p ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.65Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n3p OCA], [https://pdbe.org/7n3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n3p RCSB], [https://www.ebi.ac.uk/pdbsum/7n3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n3p ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The type V-K CRISPR-Cas system, featured by Cas12k effector with a naturally inactivated RuvC domain and associated with Tn7-like transposon for RNA-guided DNA transposition, is a promising tool for precise DNA insertion. To reveal the mechanism underlying target DNA recognition, we determined a cryoelectron microscopy (cryo-EM) structure of Cas12k from cyanobacteria Scytonema hofmanni in complex with a single guide RNA (sgRNA) and a double-stranded target DNA. Coupled with mutagenesis and in vitro DNA transposition assay, our results revealed mechanisms for the recognition of the GGTT protospacer adjacent motif (PAM) sequence and the structural elements of Cas12k critical for RNA-guided DNA transposition. These structural and mechanistic insights should aid in the development of type V-K CRISPR-transposon systems as tools for genome editing. | ||
| + | |||
| + | Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition.,Xiao R, Wang S, Han R, Li Z, Gabel C, Mukherjee IA, Chang L Mol Cell. 2021 Nov 4;81(21):4457-4466.e5. doi: 10.1016/j.molcel.2021.07.043. Epub , 2021 Aug 26. PMID:34450043<ref>PMID:34450043</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7n3p" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Z | + | [[Category: Scytonema hofmannii]] |
| + | [[Category: Chang L]] | ||
| + | [[Category: Han R]] | ||
| + | [[Category: Li Z]] | ||
| + | [[Category: Wang S]] | ||
| + | [[Category: Xiao R]] | ||
Revision as of 19:41, 29 May 2024
Cryo-EM structure of the Cas12k-sgRNA-dsDNA complex
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Categories: Large Structures | Scytonema hofmannii | Chang L | Han R | Li Z | Wang S | Xiao R
