7wax
From Proteopedia
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<StructureSection load='7wax' size='340' side='right'caption='[[7wax]], [[Resolution|resolution]] 2.35Å' scene=''> | <StructureSection load='7wax' size='340' side='right'caption='[[7wax]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[7wax]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7wax]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arsenophonus_endosymbiont_of_Nilaparvata_lugens Arsenophonus endosymbiont of Nilaparvata lugens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr> | |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wax OCA], [https://pdbe.org/7wax PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wax RCSB], [https://www.ebi.ac.uk/pdbsum/7wax PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wax ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wax OCA], [https://pdbe.org/7wax PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wax RCSB], [https://www.ebi.ac.uk/pdbsum/7wax PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wax ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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The bacterial peptidoglycan enclosing the cytoplasmic membrane is a fundamental cellular architecture. The integral membrane protein MurJ plays an essential role in flipping the cell wall building block Lipid II across the cytoplasmic membrane for peptidoglycan biosynthesis. Previously reported crystal structures of MurJ have elucidated its V-shaped inward- or outward-facing forms with an internal cavity for substrate binding. MurJ transports Lipid II using its cavity through conformational transitions between these two forms. Here, we report two crystal structures of inward-facing forms from Arsenophonus endosymbiont MurJ and an unprecedented crystal structure of Escherichia coli MurJ in a "squeezed" form, which lacks a cavity to accommodate the substrate, mainly because of the increased proximity of transmembrane helices 2 and 8. Subsequent molecular dynamics simulations supported the hypothesis that the squeezed form is an intermediate conformation. This study fills a gap in our understanding of the Lipid II flipping mechanism. | The bacterial peptidoglycan enclosing the cytoplasmic membrane is a fundamental cellular architecture. The integral membrane protein MurJ plays an essential role in flipping the cell wall building block Lipid II across the cytoplasmic membrane for peptidoglycan biosynthesis. Previously reported crystal structures of MurJ have elucidated its V-shaped inward- or outward-facing forms with an internal cavity for substrate binding. MurJ transports Lipid II using its cavity through conformational transitions between these two forms. Here, we report two crystal structures of inward-facing forms from Arsenophonus endosymbiont MurJ and an unprecedented crystal structure of Escherichia coli MurJ in a "squeezed" form, which lacks a cavity to accommodate the substrate, mainly because of the increased proximity of transmembrane helices 2 and 8. Subsequent molecular dynamics simulations supported the hypothesis that the squeezed form is an intermediate conformation. This study fills a gap in our understanding of the Lipid II flipping mechanism. | ||
| - | Crystal structure of the lipid flippase MurJ in a "squeezed" form distinct from its inward- and outward-facing forms.,Kohga H, Mori T, Tanaka Y, Yoshikaie K, Taniguchi K, Fujimoto K, Fritz L, Schneider T, Tsukazaki T Structure. 2022 | + | Crystal structure of the lipid flippase MurJ in a "squeezed" form distinct from its inward- and outward-facing forms.,Kohga H, Mori T, Tanaka Y, Yoshikaie K, Taniguchi K, Fujimoto K, Fritz L, Schneider T, Tsukazaki T Structure. 2022 Aug 4;30(8):1088-1097.e3. doi: 10.1016/j.str.2022.05.008. Epub , 2022 Jun 3. PMID:35660157<ref>PMID:35660157</ref> |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Arsenophonus endosymbiont of Nilaparvata lugens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Fujimoto | + | [[Category: Fujimoto K]] |
| - | [[Category: Kohga | + | [[Category: Kohga H]] |
| - | [[Category: Tanaka | + | [[Category: Tanaka Y]] |
| - | [[Category: Taniguchi | + | [[Category: Taniguchi K]] |
| - | [[Category: Tsukazaki | + | [[Category: Tsukazaki T]] |
| - | [[Category: Yoshikaie | + | [[Category: Yoshikaie K]] |
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Current revision
MurJ inward occluded form
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