8hg1

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[8hg1]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Monkeypox_virus Monkeypox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HG1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HG1 FirstGlance]. <br>
<table><tr><td colspan='2'>[[8hg1]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Monkeypox_virus Monkeypox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HG1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HG1 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hg1 OCA], [https://pdbe.org/8hg1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hg1 RCSB], [https://www.ebi.ac.uk/pdbsum/8hg1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hg1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hg1 OCA], [https://pdbe.org/8hg1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hg1 RCSB], [https://www.ebi.ac.uk/pdbsum/8hg1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hg1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[https://www.uniprot.org/uniprot/Q5IXS4_MONPV Q5IXS4_MONPV] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Also part of a heterodimeric processivity factor which potentiates the DNA polymerase activity. Binds to DNA.[ARBA:ARBA00025092]
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[https://www.uniprot.org/uniprot/DPOL_MONPV DPOL_MONPV] Catalyzes DNA synthesis. Acquires processivity by associating with a heterodimeric processivity factor comprised of the viral OPG148 and OPG116 proteins, thereby forming the DNA polymerase holoenzyme. Displays 3'- to 5' exonuclease activity. Might participate in viral DNA recombination. Does not perform OPG116/D4synthesis across an abasic site.[UniProtKB:P06856]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The World Health Organization declared mpox (or monkeypox) a public health emergency of international concern in July 2022, and prophylactic and therapeutic measures are in urgent need. The monkeypox virus (MPXV) has its own DNA polymerase F8, together with the processive cofactors A22 and E4, constituting the polymerase holoenzyme for genome replication. Here, we determined the holoenzyme structure in complex with DNA using cryo-electron microscopy at the global resolution of ~2.8 angstroms. The holoenzyme possesses an architecture that suggests a "forward sliding clamp" processivity mechanism for viral DNA replication. MPXV polymerase has a DNA binding mode similar to that of other B-family DNA polymerases from different species. These findings reveal the mechanism of the MPXV genome replication and may guide the development of anti-poxvirus drugs.
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Structure of monkeypox virus DNA polymerase holoenzyme.,Peng Q, Xie Y, Kuai L, Wang H, Qi J, Gao GF, Shi Y Science. 2023 Jan 6;379(6627):100-105. doi: 10.1126/science.ade6360. Epub 2022 , Dec 15. PMID:36520947<ref>PMID:36520947</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 8hg1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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</StructureSection>
</StructureSection>

Current revision

The structure of MPXV polymerase holoenzyme in replicating state

PDB ID 8hg1

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