8ilz

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the RRM domain of human SETD1B

Structural highlights

8ilz is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.77Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SET1B_HUMAN Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.

Publication Abstract from PubMed

SETD1A and SETD1B originate from Set1, the sole H3K4 methyltransferase in yeast, and they play important roles in active gene transcription. Here, we present the crystal structures of the RRM domains of human SETD1A and SETD1B. Although both RRM domains adopt a canonical RRM fold, their structural features are different from that of the yeast Set1 RRM domain, their yeast homolog. By using an ITC binding assay, we found an intrinsically disordered region in SETD1A/B binds WDR82. The structural analysis implies that the positively charged regions within human RRM domains might be involved in binding to RNA. Our work provides structural insight into the assembly of WDR82 with the catalytic subunits SETD1A/B in the context of the whole complex.

Molecular insight into the SETD1A/B N-terminal region and its interaction with WDR82.,Bao S, Xu C Biochem Biophys Res Commun. 2023 May 28;658:136-140. doi: , 10.1016/j.bbrc.2023.03.064. Epub 2023 Mar 31. PMID:37030068^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bao S, Xu C. Molecular insight into the SETD1A/B N-terminal region and its interaction with WDR82. Biochem Biophys Res Commun. 2023 May 28;658:136-140. PMID:37030068 doi:10.1016/j.bbrc.2023.03.064

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/8ilz"

Categories: Homo sapiens | Large Structures | Bao S | Xu C

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[8ilz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ILZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ILZ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ilz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ilz OCA], [https://pdbe.org/8ilz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ilz RCSB], [https://www.ebi.ac.uk/pdbsum/8ilz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ilz ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ilz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ilz OCA], [https://pdbe.org/8ilz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ilz RCSB], [https://www.ebi.ac.uk/pdbsum/8ilz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ilz ProSAT]</span></td></tr>
 </table>
 == Function ==

8ilz

From Proteopedia

Current revision

Crystal structure of the RRM domain of human SETD1B

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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