1w7g
From Proteopedia
| Line 6: | Line 6: | ||
==Overview== | ==Overview== | ||
Piperazinyl-amide derivatives of, N-alpha-(3-trifluoromethyl-benzenesulfonyl)-L-arginine (1) were, synthesized as graftable thrombin inhibitors. The possible disturbance of, biological activity due to a variable spacer-arm fixed on the N-4, piperazinyl position was evaluated in vitro, against human alpha-thrombin, and in blood coagulation assay. Molecular modelling (in silico analysis), and X-ray diffraction studies of thrombin-inhibitor complexes were also, performed. The fixation of bioactive molecules on poly(butylene, terephthalate) (PBT) and poly(ethylene terephthalate) (PET) membranes was, performed by wet chemistry treatment and evaluated by XPS analysis., Surface grafting of inhibitor 1d improved the membrane hemocompatibility, by reducing blood clot formation on the modified surface. | Piperazinyl-amide derivatives of, N-alpha-(3-trifluoromethyl-benzenesulfonyl)-L-arginine (1) were, synthesized as graftable thrombin inhibitors. The possible disturbance of, biological activity due to a variable spacer-arm fixed on the N-4, piperazinyl position was evaluated in vitro, against human alpha-thrombin, and in blood coagulation assay. Molecular modelling (in silico analysis), and X-ray diffraction studies of thrombin-inhibitor complexes were also, performed. The fixation of bioactive molecules on poly(butylene, terephthalate) (PBT) and poly(ethylene terephthalate) (PET) membranes was, performed by wet chemistry treatment and evaluated by XPS analysis., Surface grafting of inhibitor 1d improved the membrane hemocompatibility, by reducing blood clot formation on the modified surface. | ||
| + | |||
| + | ==Disease== | ||
| + | Known diseases associated with this structure: Dysprothrombinemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176930 176930]], Hyperprothrombinemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176930 176930]], Hypoprothrombinemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176930 176930]] | ||
==About this Structure== | ==About this Structure== | ||
| Line 25: | Line 28: | ||
[[Category: thrombin]] | [[Category: thrombin]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:47:27 2007'' |
Revision as of 17:41, 12 November 2007
|
ALPHA-THROMBIN COMPLEX WITH SULFATED HIRUDIN (RESIDUES 54-65) AND L-ARGININE TEMPLATE INHIBITOR CS107
Contents |
Overview
Piperazinyl-amide derivatives of, N-alpha-(3-trifluoromethyl-benzenesulfonyl)-L-arginine (1) were, synthesized as graftable thrombin inhibitors. The possible disturbance of, biological activity due to a variable spacer-arm fixed on the N-4, piperazinyl position was evaluated in vitro, against human alpha-thrombin, and in blood coagulation assay. Molecular modelling (in silico analysis), and X-ray diffraction studies of thrombin-inhibitor complexes were also, performed. The fixation of bioactive molecules on poly(butylene, terephthalate) (PBT) and poly(ethylene terephthalate) (PET) membranes was, performed by wet chemistry treatment and evaluated by XPS analysis., Surface grafting of inhibitor 1d improved the membrane hemocompatibility, by reducing blood clot formation on the modified surface.
Disease
Known diseases associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]
About this Structure
1W7G is a Protein complex structure of sequences from Homo sapiens with MIU as ligand. Active as Thrombin, with EC number 3.4.21.5 Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Design, synthesis and evaluation of graftable thrombin inhibitors for the preparation of blood-compatible polymer materials., Salvagnini C, Michaux C, Remiche J, Wouters J, Charlier P, Marchand-Brynaert J, Org Biomol Chem. 2005 Dec 7;3(23):4209-20. Epub 2005 Oct 19. PMID:16294249
Page seeded by OCA on Mon Nov 12 19:47:27 2007
Categories: Homo sapiens | Protein complex | Thrombin | Charlier, P. | Herman, R. | Remiche, J. | Sauvage, E. | MIU | Argatroban | Coagulation | Serine protease
