2ihl

From Proteopedia

(Difference between revisions)

Revision as of 05:39, 5 June 2024

LYSOZYME (E.C.3.2.1.17) (JAPANESE QUAIL)

Structural highlights

2ihl is a 1 chain structure with sequence from Coturnix japonica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.4Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LYSC_COTJA Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Although antibodies are highly specific, cross-reactions are frequently observed. To understand the molecular basis of this phenomenon, we studied the anti-hen egg lysozyme (HEL) monoclonal antibody (mAb) D11.15, which cross-reacts with several avian lysozymes, in some cases with a higher affinity (heteroclitic binding) than for HEL. We have determined the crystal structure of the Fv fragment of D11.15 complexed with pheasant egg lysozyme (PHL). In addition, we have determined the structure of PHL, Guinea fowl egg lysozyme, and Japanese quail egg lysozyme. Differences in the affinity of D11.15 for the lysozymes appear to result from sequence substitutions in these antigens at the interface with the antibody. More generally, cross-reactivity is seen to require a stereochemically permissive environment for the variant antigen residues at the antibody-antigen interface.

Three-dimensional structure of a heteroclitic antigen-antibody cross-reaction complex.,Chitarra V, Alzari PM, Bentley GA, Bhat TN, Eisele JL, Houdusse A, Lescar J, Souchon H, Poljak RJ Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7711-5. PMID:8356074^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chitarra V, Alzari PM, Bentley GA, Bhat TN, Eisele JL, Houdusse A, Lescar J, Souchon H, Poljak RJ. Three-dimensional structure of a heteroclitic antigen-antibody cross-reaction complex. Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7711-5. PMID:8356074

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ihl"

@@ Line 3: / Line 3: @@
 <StructureSection load='2ihl' size='340' side='right'caption='[[2ihl]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ihl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cotja Cotja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IHL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ihl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Coturnix_japonica Coturnix japonica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IHL FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ihl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ihl OCA], [https://pdbe.org/2ihl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ihl RCSB], [https://www.ebi.ac.uk/pdbsum/2ihl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ihl ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/LYSC_COTJA LYSC_COTJA]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
+[https://www.uniprot.org/uniprot/LYSC_COTJA LYSC_COTJA] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 36: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Cotja]]
+[[Category: Coturnix japonica]]
 [[Category: Large Structures]]
-[[Category: Lysozyme]]
+[[Category: Bentley GA]]
-[[Category: Bentley, G A]]
+[[Category: Houdusse A]]
-[[Category: Houdusse, A]]
+[[Category: Poljak RJ]]
-[[Category: Poljak, R J]]
+[[Category: Souchon H]]
-[[Category: Souchon, H]]
+[[Category: Zhang Z]]
-[[Category: Zhang, Z]]

2ihl

From Proteopedia

Revision as of 05:39, 5 June 2024

LYSOZYME (E.C.3.2.1.17) (JAPANESE QUAIL)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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