7eu1
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==The cryo-EM structure of A. thaliana Pol IV-RDR2 holoenzyme== |
| - | <StructureSection load='7eu1' size='340' side='right'caption='[[7eu1]]' scene=''> | + | <StructureSection load='7eu1' size='340' side='right'caption='[[7eu1]], [[Resolution|resolution]] 3.86Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7eu1]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EU1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EU1 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eu1 OCA], [https://pdbe.org/7eu1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eu1 RCSB], [https://www.ebi.ac.uk/pdbsum/7eu1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eu1 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.86Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eu1 OCA], [https://pdbe.org/7eu1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eu1 RCSB], [https://www.ebi.ac.uk/pdbsum/7eu1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eu1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NRPBC_ARATH NRPBC_ARATH] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. Component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements.<ref>PMID:19110459</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA methylation affects gene expression and maintains genome integrity. The DNA-dependent RNA polymerase IV (Pol IV), together with the RNA-dependent RNA polymerase RDR2, produces double-stranded small interfering RNA precursors essential for establishing and maintaining DNA methylation in plants. We determined the cryo-electron microscopy structures of the Pol IV-RDR2 holoenzyme and the backtracked transcription elongation complex. These structures reveal that Pol IV and RDR2 form a complex with their active sites connected by an interpolymerase channel, through which the Pol IV-generated transcript is handed over to the RDR2 active site after being backtracked, where it is used as the template for double-stranded RNA (dsRNA) synthesis. Our results describe a 'backtracking-triggered RNA channeling' mechanism underlying dsRNA synthesis and also shed light on the evolutionary trajectory of eukaryotic RNA polymerases. | ||
| + | |||
| + | Pol IV and RDR2: A two-RNA-polymerase machine that produces double-stranded RNA.,Huang K, Wu XX, Fang CL, Xu ZG, Zhang HW, Gao J, Zhou CM, You LL, Gu ZX, Mu WH, Feng Y, Wang JW, Zhang Y Science. 2021 Dec 24;374(6575):1579-1586. doi: 10.1126/science.abj9184. Epub 2021 , Dec 23. PMID:34941388<ref>PMID:34941388</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7eu1" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Arabidopsis thaliana]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Fang CL]] |
| + | [[Category: Huang K]] | ||
| + | [[Category: Wu XX]] | ||
| + | [[Category: Zhang Y]] | ||
Current revision
The cryo-EM structure of A. thaliana Pol IV-RDR2 holoenzyme
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