7sfs
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7sfs]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_virus_Sf6 Shigella virus Sf6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SFS FirstGlance]. <br> | <table><tr><td colspan='2'>[[7sfs]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_virus_Sf6 Shigella virus Sf6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SFS FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sfs OCA], [https://pdbe.org/7sfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sfs RCSB], [https://www.ebi.ac.uk/pdbsum/7sfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sfs ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.76Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sfs OCA], [https://pdbe.org/7sfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sfs RCSB], [https://www.ebi.ac.uk/pdbsum/7sfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sfs ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/Q716H2_BPSFV Q716H2_BPSFV] | [https://www.uniprot.org/uniprot/Q716H2_BPSFV Q716H2_BPSFV] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sf6 is a bacterial virus that infects the human pathogen Shigella flexneri. Here, we describe the cryo-electron microscopy structure of the Sf6 tail machine before DNA ejection, which we determined at a 2.7-angstrom resolution. We built de novo structures of all tail components and resolved four symmetry-mismatched interfaces. Unexpectedly, we found that the tail exists in two conformations, rotated by ~6 degrees with respect to the capsid. The two tail conformers are identical in structure but differ solely in how the portal and head-to-tail adaptor carboxyl termini bond with the capsid at the fivefold vertex, similar to a diamond held over a five-pronged ring in two nonidentical states. Thus, in the mature Sf6 tail, the portal structure does not morph locally to accommodate the symmetry mismatch but exists in two energetic minima rotated by a discrete angle. We propose that the design principles of the Sf6 tail are conserved across P22-like Podoviridae. | ||
| + | |||
| + | High-resolution cryo-EM structure of the Shigella virus Sf6 genome delivery tail machine.,Li F, Hou CD, Yang R, Whitehead R 3rd, Teschke CM, Cingolani G Sci Adv. 2022 Dec 9;8(49):eadc9641. doi: 10.1126/sciadv.adc9641. Epub 2022 Dec 7. PMID:36475795<ref>PMID:36475795</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7sfs" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
In situ cryo-EM structure of bacteriophage Sf6 portal:gp7 complex at 2.7A resolution
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