8s1w
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8s1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8s1w OCA], [https://pdbe.org/8s1w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8s1w RCSB], [https://www.ebi.ac.uk/pdbsum/8s1w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8s1w ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8s1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8s1w OCA], [https://pdbe.org/8s1w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8s1w RCSB], [https://www.ebi.ac.uk/pdbsum/8s1w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8s1w ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A guanine-rich oligonucleotide based on a human telomeric sequence but with the first three-nucleotide intervening stretch replaced by a putative 15-nucleotide hairpin-forming sequence shows a pH-dependent folding into different quadruplex-duplex hybrids in a potassium containing buffer. At slightly acidic pH, the quadruplex domain adopts a chair-type conformation. Upon increasing the pH, a transition with a midpoint close to neutral pH to a major and minor (3+1) hybrid topology with either a coaxially stacked or orthogonally oriented duplex stem-loop occurs. NMR-derived high-resolution structures reveal that an adenine protonation is prerequisite for the formation of a non-canonical base quartet, capping the outer G-tetrad at the quadruplex-duplex interface and stabilizing the antiparallel chair conformation in an acidic environment. Being directly associated with interactions at the quadruplex-duplex interface, this unique pH-dependent topological transition is fully reversible. Coupled with a conformation-sensitive optical readout demonstrated as a proof of concept using the fluorescent dye thiazole orange, the present quadruplex-duplex hybrid architecture represents a potentially valuable pH-sensing system responsive in a physiological pH range of 7+/-1. | ||
| + | |||
| + | A pH-Responsive Topological Switch Based on a DNA Quadruplex-Duplex Hybrid.,Vianney YM, Jana J, Weisz K Chemistry. 2024 May 23;30(29):e202400722. doi: 10.1002/chem.202400722. Epub 2024 , Apr 9. PMID:38497675<ref>PMID:38497675</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8s1w" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Structure of a quadruplex-duplex hybrid with a (-pd+l) loop progression
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