1snl
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1snl.gif|left|200px]] | [[Image:1snl.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1snl", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1snl| PDB=1snl | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''NMR Solution Structure of the Calcium-binding Domain of Nucleobindin (CALNUC)''' | '''NMR Solution Structure of the Calcium-binding Domain of Nucleobindin (CALNUC)''' | ||
| Line 27: | Line 24: | ||
[[Category: Alba, E de.]] | [[Category: Alba, E de.]] | ||
[[Category: Tjandra, N.]] | [[Category: Tjandra, N.]] | ||
| - | [[Category: | + | [[Category: Calcium-binding]] |
| - | [[Category: | + | [[Category: Ef-hand]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:55:39 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:55, 3 May 2008
NMR Solution Structure of the Calcium-binding Domain of Nucleobindin (CALNUC)
Overview
Nucleobindin, also known as calnuc, participates in Ca2+ storage in the Golgi, as well as in other biological processes that involve DNA-binding and protein-protein interactions. We have determined the three-dimensional solution structure of the Ca(2+)-binding domain of nucleobindin by NMR showing that it consists of two EF-hand motifs. The NMR structure indicates that the phi and psi angles of residues in both motifs are very similar, despite the noncanonical sequence of the C-terminal EF-hand, which contains an arginine residue instead of the typical glycine at the sixth position of the 12-residue loop. The relative orientation of the alpha-helices in the N-terminal EF-hand falls within the common arrangement found in most EF-hand structures. In contrast, the noncanonical EF-hand deviates from the average orientation. The two helix-loop-helix moieties are in the open conformation characteristic of the Ca(2+)-bound state. We find that both motifs bind Ca2+ with apparent dissociation constants of 47 and 40 microM for the noncanonical and the canonical EF-hand, respectively. The Ca(2+)-binding domain of nucleobindin is unstructured in the absence of Ca2+ and folds upon Ca2+ addition. NMR relaxation data and structural studies of the folded domain indicate that it undergoes slow dynamics, suggesting that it is floppier and less compact than a globular domain.
About this Structure
1SNL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural studies on the Ca2+-binding domain of human nucleobindin (calnuc)., de Alba E, Tjandra N, Biochemistry. 2004 Aug 10;43(31):10039-49. PMID:15287731 Page seeded by OCA on Sat May 3 08:55:39 2008
