7ujl
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7ujl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UJL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UJL FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ujl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UJL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UJL FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ujl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ujl OCA], [https://pdbe.org/7ujl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ujl RCSB], [https://www.ebi.ac.uk/pdbsum/7ujl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ujl ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ujl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ujl OCA], [https://pdbe.org/7ujl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ujl RCSB], [https://www.ebi.ac.uk/pdbsum/7ujl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ujl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == Function == | ||
| - | [https://www.uniprot.org/uniprot/VBET_LAMBD VBET_LAMBD] Gene bet protein functions in general recombination and in the late, rolling-circle mode of lambda DNA replication. Has a function similar to that of E.coli recT. It is a single-stranded DNA binding protein that can promote renaturation of DNA. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
The Redbeta protein of the bacteriophage lambda red recombination system is a model annealase which catalyzes single-strand annealing homologous DNA recombination. Here we present the structure of a helical oligomeric annealing intermediate of Redbeta, consisting of N-terminal residues 1-177 bound to two complementary 27mer oligonucleotides, determined via cryogenic electron microscopy (cryo-EM) to a final resolution of 3.3 A. The structure reveals a continuous binding groove which positions and stabilizes complementary DNA strands in a planar orientation to facilitate base pairing via a network of hydrogen bonding. Definition of the inter-subunit interface provides a structural basis for the propensity of Redbeta to oligomerize into functionally significant long helical filaments, a trait shared by most annealases. Our cryo-EM structure and molecular dynamics simulations suggest that residues 133-138 form a flexible loop which modulates access to the binding groove. More than half a century after its discovery, this combination of structural and computational observations has allowed us to propose molecular mechanisms for the actions of the model annealase Redbeta, a defining member of the Redbeta/RecT protein family. | The Redbeta protein of the bacteriophage lambda red recombination system is a model annealase which catalyzes single-strand annealing homologous DNA recombination. Here we present the structure of a helical oligomeric annealing intermediate of Redbeta, consisting of N-terminal residues 1-177 bound to two complementary 27mer oligonucleotides, determined via cryogenic electron microscopy (cryo-EM) to a final resolution of 3.3 A. The structure reveals a continuous binding groove which positions and stabilizes complementary DNA strands in a planar orientation to facilitate base pairing via a network of hydrogen bonding. Definition of the inter-subunit interface provides a structural basis for the propensity of Redbeta to oligomerize into functionally significant long helical filaments, a trait shared by most annealases. Our cryo-EM structure and molecular dynamics simulations suggest that residues 133-138 form a flexible loop which modulates access to the binding groove. More than half a century after its discovery, this combination of structural and computational observations has allowed us to propose molecular mechanisms for the actions of the model annealase Redbeta, a defining member of the Redbeta/RecT protein family. | ||
| - | + | Redbeta(177) annealase structure reveals details of oligomerization and lambda Red-mediated homologous DNA recombination.,Newing TP, Brewster JL, Fitschen LJ, Bouwer JC, Johnston NP, Yu H, Tolun G Nat Commun. 2022 Sep 26;13(1):5649. doi: 10.1038/s41467-022-33090-6. PMID:36163171<ref>PMID:36163171</ref> | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
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[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Newing TP]] | [[Category: Newing TP]] | ||
| - | [[Category: Tolun | + | [[Category: Tolun G]] |
Current revision
Bacteriophage Lambda Red-Beta N-terminal domain helical assembly in complex with dsDNA
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