8dq0

From Proteopedia

(Difference between revisions)

Current revision

Quorum-sensing receptor RhlR bound to PqsE

Structural highlights

8dq0 is a 4 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.74Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RHLR_PSEAE Quorum-sensing regulator that controls the expression of multiple virulence factors in response to extracellular signaling molecules called autoinducers (PubMed:15576196, PubMed:7494482, PubMed:8522523). Involved, among others, in the transcriptional regulation of genes that are responsible for rhamnolipid surfactant biosynthesis (PubMed:14526008, PubMed:7604006, PubMed:8144472). Acts by binding to a specific sequence in the rhlAB regulatory region, both in the presence and in the absence of its autoinducer (PubMed:14526008). In the former case it activates transcription of the promoter, whereas in the latter it acts as a transcriptional repressor (PubMed:14526008). Also regulates the expression of the rmlBDAC operon, encoding dTDP-L-rhamnose biosynthetic enzymes, by binding to the rml box in the promoter region (PubMed:22262098). In addition, is involved in the regulation of the production of elastase (lasB) and pyocyanine (PubMed:7604006, PubMed:8144472, PubMed:8522523).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

Publication Abstract from PubMed

Pseudomonas aeruginosa is an opportunistic pathogen that is responsible for thousands of deaths every year in the United States. P. aeruginosa virulence factor production is mediated by quorum sensing, a mechanism of bacterial cell-cell communication that relies on the production and detection of signal molecules called autoinducers. In P. aeruginosa, the transcription factor receptor RhlR is activated by a RhlI-synthesized autoinducer. We recently showed that RhlR-dependent transcription is enhanced by a physical interaction with the enzyme PqsE via increased affinity of RhlR for promoter DNA. However, the molecular basis for complex formation and how complex formation enhanced RhlR transcriptional activity remained unclear. Here, we report the structure of ligand-bound RhlR in complex with PqsE. Additionally, we determined the structure of the complex bound with DNA, revealing the mechanism by which RhlR-mediated transcription is enhanced by PqsE, thereby establishing the molecular basis for RhlR-dependent virulence factor production in P. aeruginosa.

Structure of the RhlR-PqsE complex from Pseudomonas aeruginosa reveals mechanistic insights into quorum-sensing gene regulation.,Feathers JR, Richael EK, Simanek KA, Fromme JC, Paczkowski JE Structure. 2022 Dec 1;30(12):1626-1636.e4. doi: 10.1016/j.str.2022.10.008. Epub , 2022 Nov 14. PMID:36379213^[8]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Medina G, Juarez K, Valderrama B, Soberon-Chavez G. Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. J Bacteriol. 2003 Oct;185(20):5976-83. doi: 10.1128/JB.185.20.5976-5983.2003. PMID:14526008 doi:http://dx.doi.org/10.1128/JB.185.20.5976-5983.2003
↑ Wagner VE, Gillis RJ, Iglewski BH. Transcriptome analysis of quorum-sensing regulation and virulence factor expression in Pseudomonas aeruginosa. Vaccine. 2004 Dec 6;22 Suppl 1:S15-20. doi: 10.1016/j.vaccine.2004.08.011. PMID:15576196 doi:http://dx.doi.org/10.1016/j.vaccine.2004.08.011
↑ Aguirre-Ramirez M, Medina G, Gonzalez-Valdez A, Grosso-Becerra V, Soberon-Chavez G. The Pseudomonas aeruginosa rmlBDAC operon, encoding dTDP-L-rhamnose biosynthetic enzymes, is regulated by the quorum-sensing transcriptional regulator RhlR and the alternative sigma factor sigmaS. Microbiology (Reading). 2012 Apr;158(Pt 4):908-916. doi: 10.1099/mic.0.054726-0. , Epub 2012 Jan 19. PMID:22262098 doi:http://dx.doi.org/10.1099/mic.0.054726-0
↑ Latifi A, Winson MK, Foglino M, Bycroft BW, Stewart GS, Lazdunski A, Williams P. Multiple homologues of LuxR and LuxI control expression of virulence determinants and secondary metabolites through quorum sensing in Pseudomonas aeruginosa PAO1. Mol Microbiol. 1995 Jul;17(2):333-43. doi: , 10.1111/j.1365-2958.1995.mmi_17020333.x. PMID:7494482 doi:http://dx.doi.org/10.1111/j.1365-2958.1995.mmi_17020333.x
↑ Ochsner UA, Reiser J. Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6424-8. PMID:7604006
↑ Ochsner UA, Koch AK, Fiechter A, Reiser J. Isolation and characterization of a regulatory gene affecting rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa. J Bacteriol. 1994 Apr;176(7):2044-54. doi: 10.1128/jb.176.7.2044-2054.1994. PMID:8144472 doi:http://dx.doi.org/10.1128/jb.176.7.2044-2054.1994
↑ Brint JM, Ohman DE. Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family. J Bacteriol. 1995 Dec;177(24):7155-63. PMID:8522523
↑ Feathers JR, Richael EK, Simanek KA, Fromme JC, Paczkowski JE. Structure of the RhlR-PqsE complex from Pseudomonas aeruginosa reveals mechanistic insights into quorum-sensing gene regulation. Structure. 2022 Dec 1;30(12):1626-1636.e4. doi: 10.1016/j.str.2022.10.008. Epub , 2022 Nov 14. PMID:36379213 doi:http://dx.doi.org/10.1016/j.str.2022.10.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/8dq0"

Categories: Large Structures | Pseudomonas aeruginosa | Feathers JR | Fromme JC | Paczkowski JE

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[8dq0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DQ0 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K5G:4-(3-bromophenoxy)-N-[(3S)-2-oxothiolan-3-yl]butanamide'>K5G</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.74&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K5G:4-(3-bromophenoxy)-N-[(3S)-2-oxothiolan-3-yl]butanamide'>K5G</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dq0 OCA], [https://pdbe.org/8dq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dq0 RCSB], [https://www.ebi.ac.uk/pdbsum/8dq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dq0 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[https://www.uniprot.org/uniprot/A9JPX4_PSEAI A9JPX4_PSEAI]
+[https://www.uniprot.org/uniprot/RHLR_PSEAE RHLR_PSEAE] Quorum-sensing regulator that controls the expression of multiple virulence factors in response to extracellular signaling molecules called autoinducers (PubMed:15576196, PubMed:7494482, PubMed:8522523). Involved, among others, in the transcriptional regulation of genes that are responsible for rhamnolipid surfactant biosynthesis (PubMed:14526008, PubMed:7604006, PubMed:8144472). Acts by binding to a specific sequence in the rhlAB regulatory region, both in the presence and in the absence of its autoinducer (PubMed:14526008). In the former case it activates transcription of the promoter, whereas in the latter it acts as a transcriptional repressor (PubMed:14526008). Also regulates the expression of the rmlBDAC operon, encoding dTDP-L-rhamnose biosynthetic enzymes, by binding to the rml box in the promoter region (PubMed:22262098). In addition, is involved in the regulation of the production of elastase (lasB) and pyocyanine (PubMed:7604006, PubMed:8144472, PubMed:8522523).<ref>PMID:14526008</ref> <ref>PMID:15576196</ref> <ref>PMID:22262098</ref> <ref>PMID:7494482</ref> <ref>PMID:7604006</ref> <ref>PMID:8144472</ref> <ref>PMID:8522523</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 8dq0" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Thioesterase 3D structures|Thioesterase 3D structures]]
 == References ==
 <references/>

8dq0

From Proteopedia

Current revision

Quorum-sensing receptor RhlR bound to PqsE

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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