1sq6
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1sq6.gif|left|200px]] | [[Image:1sq6.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1sq6", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1sq6| PDB=1sq6 | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Plasmodium falciparum homolog of Uridine phosphorylase/Purine nucleoside phosphorylase''' | '''Plasmodium falciparum homolog of Uridine phosphorylase/Purine nucleoside phosphorylase''' | ||
| Line 29: | Line 26: | ||
[[Category: Robien, M A.]] | [[Category: Robien, M A.]] | ||
[[Category: SGPP, Structural Genomics of Pathogenic Protozoa Consortium.]] | [[Category: SGPP, Structural Genomics of Pathogenic Protozoa Consortium.]] | ||
| - | [[Category: | + | [[Category: Alpha+beta]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Sgpp]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Structural genomics of pathogenic protozoa consortium]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:00:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:00, 3 May 2008
Plasmodium falciparum homolog of Uridine phosphorylase/Purine nucleoside phosphorylase
Overview
Purine metabolism in the parasite Plasmodium has been identified as a promising target for antimalarial therapies. Purine nucleoside phosphorylase (PNP) is part of a salvage pathway for the biosynthesis of purines, which are essential for parasite survival. Two crystal structures of PNP from Plasmodium falciparum (PfPNP) in two space groups, each with a single subunit in the asymmetric unit, are described here. One structure, refined to 2.4 A, has an empty nucleoside-binding site and a sulfate ion bound in the phosphate-binding pocket. The second structure, refined to 2.0 A, has the substrate inosine bound to the active centre. Structure comparison reveals alterations in the active site upon ligand binding. The new structures presented here specifically highlight the likely roles of Asp206 and two loops flanking the active site: the beta7-alpha6 loop (residues approximately 161-169) and the beta9-alpha8 loop (residues approximately 208-223). Comparison with PNP in complex with transition-state inhibitors suggests that the purine substrate moves towards the phosphate substrate, rather than vice versa, upon forming the transition state. The single-substrate-containing PfPNP structures also appear to be more flexible than PfPNP bound to inhibitors. Together, these structures serve as a basis for better understanding of ligand binding and mechanism that can be further exploited to optimize the specificity of anti-PfPNP drugs.
About this Structure
1SQ6 is a Single protein structure of sequence from Plasmodium falciparum 3d7. Full crystallographic information is available from OCA.
Reference
Structures of Plasmodium falciparum purine nucleoside phosphorylase complexed with sulfate and its natural substrate inosine., Schnick C, Robien MA, Brzozowski AM, Dodson EJ, Murshudov GN, Anderson L, Luft JR, Mehlin C, Hol WG, Brannigan JA, Wilkinson AJ, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1245-54. Epub 2005, Aug 16. PMID:16131758 Page seeded by OCA on Sat May 3 09:00:29 2008
Categories: Plasmodium falciparum 3d7 | Single protein | Uridine phosphorylase | Hol, W G.J. | Robien, M A. | SGPP, Structural Genomics of Pathogenic Protozoa Consortium. | Alpha+beta | Protein structure initiative | Psi | Sgpp | Structural genomic | Structural genomics of pathogenic protozoa consortium
