6fok

From Proteopedia

(Difference between revisions)

Current revision

Copper transporter OprC

Structural highlights

6fok is a 2 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.97Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

G3XD89_PSEAE

Publication Abstract from PubMed

Copper, while toxic in excess, is an essential micronutrient in all kingdoms of life due to its essential role in the structure and function of many proteins. Proteins mediating ionic copper import have been characterised in detail for eukaryotes, but much less so for prokaryotes. In particular, it is still unclear whether and how gram-negative bacteria acquire ionic copper. Here, we show that Pseudomonas aeruginosa OprC is an outer membrane, TonB-dependent transporter that is conserved in many Proteobacteria and which mediates acquisition of both reduced and oxidised ionic copper via an unprecedented CxxxM-HxM metal binding site. Crystal structures of wild-type and mutant OprC variants with silver and copper suggest that acquisition of Cu(I) occurs via a surface-exposed "methionine track" leading towards the principal metal binding site. Together with whole-cell copper quantitation and quantitative proteomics in a murine lung infection model, our data identify OprC as an abundant component of bacterial copper biology that may enable copper acquisition under a wide range of conditions.

Acquisition of ionic copper by the bacterial outer membrane protein OprC through a novel binding site.,Bhamidimarri SP, Young TR, Shanmugam M, Soderholm S, Basle A, Bumann D, van den Berg B PLoS Biol. 2021 Nov 11;19(11):e3001446. doi: 10.1371/journal.pbio.3001446., eCollection 2021 Nov. PMID:34762655^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bhamidimarri SP, Young TR, Shanmugam M, Soderholm S, Basle A, Bumann D, van den Berg B. Acquisition of ionic copper by the bacterial outer membrane protein OprC through a novel binding site. PLoS Biol. 2021 Nov 11;19(11):e3001446. doi: 10.1371/journal.pbio.3001446., eCollection 2021 Nov. PMID:34762655 doi:http://dx.doi.org/10.1371/journal.pbio.3001446

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6fok"

Categories: Large Structures | Pseudomonas aeruginosa PAO1 | Bhamidimarri SP | Van den Berg B

@@ Line 3: / Line 3: @@
 <StructureSection load='6fok' size='340' side='right'caption='[[6fok]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6fok]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FOK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6fok]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FOK FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6fom|6fom]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">oprC, PA3790 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fok OCA], [https://pdbe.org/6fok PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fok RCSB], [https://www.ebi.ac.uk/pdbsum/6fok PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fok ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/G3XD89_PSEAE G3XD89_PSEAE]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Pseae]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-[[Category: Berg, B van den]]
+[[Category: Bhamidimarri SP]]
-[[Category: Bhamidimarri, S P]]
+[[Category: Van den Berg B]]
-[[Category: Copper]]
-[[Category: Membrane protein]]
-[[Category: Metalloprotein]]
-[[Category: Outer membrane protein]]
-[[Category: Pseudomonas aeruginosa]]
-[[Category: Tonb dependent transporter]]

6fok

From Proteopedia

Current revision

Copper transporter OprC

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox