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| | <StructureSection load='7b73' size='340' side='right'caption='[[7b73]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='7b73' size='340' side='right'caption='[[7b73]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7b73]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gamma_proteobacterium_htcc2207 Gamma proteobacterium htcc2207]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B73 FirstGlance]. <br> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B73 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GB2207_06463 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=314287 gamma proteobacterium HTCC2207])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b73 OCA], [https://pdbe.org/7b73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b73 RCSB], [https://www.ebi.ac.uk/pdbsum/7b73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b73 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b73 OCA], [https://pdbe.org/7b73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b73 RCSB], [https://www.ebi.ac.uk/pdbsum/7b73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b73 ProSAT]</span></td></tr> |
| | </table> | | </table> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Gamma proteobacterium htcc2207]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bosh, S]] | + | [[Category: Bosh S]] |
| - | [[Category: Dijkman, W P]] | + | [[Category: Dijkman WP]] |
| - | [[Category: Estevez, M]] | + | [[Category: Estevez M]] |
| - | [[Category: Hidalgo, A]] | + | [[Category: Hidalgo A]] |
| - | [[Category: Osuna, S]] | + | [[Category: Osuna S]] |
| - | [[Category: Petrillo, G]] | + | [[Category: Petrillo G]] |
| - | [[Category: Schallmey, A]] | + | [[Category: Schallmey A]] |
| - | [[Category: Seeger, M]] | + | [[Category: Seeger M]] |
| - | [[Category: Uson, I]] | + | [[Category: Uson I]] |
| - | [[Category: Wessel, J]] | + | [[Category: Wessel J]] |
| - | [[Category: Dehalogenase]]
| + | |
| - | [[Category: Directed evolution]]
| + | |
| - | [[Category: Enantioselectivity change]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Protein engineering]]
| + | |
| - | [[Category: Short-chain dehydrogenase/reductase enzyme superfamily]]
| + | |
| - | [[Category: Thermostability]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Halohydrin dehalogenases (HHDHs) are promising enzymes for application in biocatalysis due to their promiscuous epoxide ring-opening activity with various anionic nucleophiles. So far, seven different HHDH subtypes A to G have been reported with subtype D containing the by far largest number of enzymes. Moreover, several characterized members of subtype D have been reported to display outstanding characteristics such as high catalytic activity, broad substrate spectra or remarkable thermal stability. Yet, no structure of a D-type HHDH has been reported to date that could be used to investigate and understand those features on a molecular level. We therefore solved the crystal structure of HheD2 from gamma proteobacterium HTCC2207 at 1.6 A resolution and used it as a starting point for targeted mutagenesis in combination with molecular dynamics (MD) simulation, in order to study the low thermal stability of HheD2 in comparison with other members of subtype D. This revealed a hydrogen bond between conserved residues Q160 and D198 to be connected with a high catalytic activity of this enzyme. Moreover, a flexible surface region containing two alpha-helices was identified to impact thermal stability of HheD2. Exchange of this surface region by residues of HheD3 yielded a variant with 10 degrees C higher melting temperature and reaction temperature optimum. Overall, our results provide important insights into the structure-function relationship of HheD2 and presumably for other D-type HHDHs. DATABASES: Structural data are available in PDB database under the accession number 7B73.
Insights into the molecular determinants of thermal stability in halohydrin dehalogenase HheD2.,Wessel J, Petrillo G, Estevez-Gay M, Bosch S, Seeger M, Dijkman WP, Iglesias-Fernandez J, Hidalgo A, Uson I, Osuna S, Schallmey A FEBS J. 2021 Feb 19. doi: 10.1111/febs.15777. PMID:33605544[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wessel J, Petrillo G, Estevez-Gay M, Bosch S, Seeger M, Dijkman WP, Iglesias-Fernandez J, Hidalgo A, Uson I, Osuna S, Schallmey A. Insights into the molecular determinants of thermal stability in halohydrin dehalogenase HheD2. FEBS J. 2021 Feb 19. doi: 10.1111/febs.15777. PMID:33605544 doi:http://dx.doi.org/10.1111/febs.15777
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