7nfu

<table><tr><td colspan='2'>[[7nfu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_thermoleovorans_ccb_us3_uf5 Geobacillus thermoleovorans ccb_us3_uf5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NFU FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">noc, GTCCBUS3UF5_39100 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111068 Geobacillus thermoleovorans CCB_US3_UF5])</td></tr>

== Function ==

[[https://www.uniprot.org/uniprot/G8N1K9_GEOTH G8N1K9_GEOTH]] Effects nucleoid occlusion by binding relatively nonspecifically to DNA and preventing the assembly of the division machinery in the vicinity of the nucleoid, especially under conditions that disturb the cell cycle. It helps to coordinate cell division and chromosome segregation by preventing the formation of the Z ring through the nucleoid, which would cause chromosome breakage.[HAMAP-Rule:MF_02015]

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== Publication Abstract from PubMed ==

ATP- and GTP-dependent molecular switches are extensively used to control functions of proteins in a wide range of biological processes. However, CTP switches are rarely reported. Here, we report that a nucleoid occlusion protein Noc is a CTPase enzyme whose membrane-binding activity is directly regulated by a CTP switch. In Bacillus subtilis, Noc nucleates on 16 bp NBS sites before associating with neighboring non-specific DNA to form large membrane-associated nucleoprotein complexes to physically occlude assembly of the cell division machinery. By in vitro reconstitution, we show that (1) CTP is required for Noc to form the NBS-dependent nucleoprotein complex, and (2) CTP binding, but not hydrolysis, switches Noc to a membrane-active state. Overall, we suggest that CTP couples membrane-binding activity of Noc to nucleoprotein complex formation to ensure productive recruitment of DNA to the bacterial cell membrane for nucleoid occlusion activity.

CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc.,Jalal ASB, Tran NT, Wu LJ, Ramakrishnan K, Rejzek M, Gobbato G, Stevenson CEM, Lawson DM, Errington J, Le TBK Mol Cell. 2021 Jul 9. pii: S1097-2765(21)00505-0. doi:, 10.1016/j.molcel.2021.06.025. PMID:34270916<ref>PMID:34270916</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

<references/>

[[Category: Geobacillus thermoleovorans ccb_us3_uf5]]

[[Category: Errington, J]]

[[Category: Jalal, A S.B]]

[[Category: Lawson, D M]]

[[Category: Le, T B.K]]

[[Category: Ramakrishnan, K]]

[[Category: Rejzek, M]]

[[Category: Stevenson, C E.M]]

[[Category: Tran, N T]]

[[Category: Wu, L J]]

[[Category: Protein-dna recognition]]