8fe3

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==Structure of dengue virus (DENV2) in complex with prM12, an anti-PrM monoclonal antibody==
==Structure of dengue virus (DENV2) in complex with prM12, an anti-PrM monoclonal antibody==
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<StructureSection load='8fe3' size='340' side='right'caption='[[8fe3]], [[Resolution|resolution]] 10.20&Aring;' scene=''>
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<SX load='8fe3' size='340' side='right' viewer='molstar' caption='[[8fe3]], [[Resolution|resolution]] 10.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[8fe3]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FE3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FE3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[8fe3]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FE3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FE3 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fe3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fe3 OCA], [https://pdbe.org/8fe3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fe3 RCSB], [https://www.ebi.ac.uk/pdbsum/8fe3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fe3 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fe3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fe3 OCA], [https://pdbe.org/8fe3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fe3 RCSB], [https://www.ebi.ac.uk/pdbsum/8fe3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fe3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[https://www.uniprot.org/uniprot/A0A481XTV0_9FLAV A0A481XTV0_9FLAV] Component of the viral RNA replication complex that functions in virion assembly and antagonizes the host immune response.[ARBA:ARBA00024317] Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[ARBA:ARBA00003504]
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[https://www.uniprot.org/uniprot/E7FLK7_9FLAV E7FLK7_9FLAV]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cleavage of the flavivirus premembrane (prM) structural protein during maturation can be inefficient. The contribution of partially mature flavivirus virions that retain uncleaved prM to pathogenesis during primary infection is unknown. To investigate this question, we characterized the functional properties of newly-generated dengue virus (DENV) prM-reactive monoclonal antibodies (mAbs) in vitro and using a mouse model of DENV disease. Anti-prM mAbs neutralized DENV infection in a virion maturation state-dependent manner. Alanine scanning mutagenesis and cryoelectron microscopy of anti-prM mAbs in complex with immature DENV defined two modes of attachment to a single antigenic site. In vivo, passive transfer of intact anti-prM mAbs resulted in an antibody-dependent enhancement of disease. However, protection against DENV-induced lethality was observed when the transferred mAbs were genetically modified to inhibit their ability to interact with Fcgamma receptors. These data establish that in addition to mature forms of the virus, partially mature infectious prM(+) virions can also contribute to pathogenesis during primary DENV infections.
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prM-reactive antibodies reveal a role for partially mature virions in dengue virus pathogenesis.,A Dowd K, Sirohi D, D Speer S, VanBlargan LA, Chen RE, Mukherjee S, Whitener BM, Govero J, Aleshnick M, Larman B, Sukupolvi-Petty S, Sevvana M, Miller AS, Klose T, Zheng A, Koenig S, Kielian M, Kuhn RJ, Diamond MS, Pierson TC Proc Natl Acad Sci U S A. 2023 Jan 17;120(3):e2218899120. doi: , 10.1073/pnas.2218899120. Epub 2023 Jan 13. PMID:36638211<ref>PMID:36638211</ref>
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 8fe3" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
[[Category: Dengue virus 2]]
[[Category: Dengue virus 2]]
[[Category: Large Structures]]
[[Category: Large Structures]]

Current revision

Structure of dengue virus (DENV2) in complex with prM12, an anti-PrM monoclonal antibody

8fe3, resolution 10.20Å

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